Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 38496..39031 Replicon plasmid pU90-4
Accession NZ_CP068039
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag JKF55_RS25745 Protein ID WP_001270417.1
Coordinates 38496..38783 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag JKF55_RS25750 Protein ID WP_001132895.1
Coordinates 38780..39031 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS25720 34041..34150 + 110 Protein_35 IS5/IS1182 family transposase -
JKF55_RS25725 34673..34923 + 251 Protein_36 type II toxin-antitoxin system ParD family antitoxin -
JKF55_RS25730 35567..36307 + 741 WP_001066942.1 tyrosine-type recombinase/integrase -
JKF55_RS25735 36592..37569 - 978 WP_000361610.1 RepB family plasmid replication initiator protein -
JKF55_RS25740 38249..38497 - 249 Protein_39 transposase -
JKF55_RS25745 38496..38783 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JKF55_RS25750 38780..39031 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JKF55_RS25755 39204..39416 - 213 WP_000402944.1 hypothetical protein -
JKF55_RS25760 39790..40995 + 1206 WP_000817634.1 AAA family ATPase -
JKF55_RS25765 40992..41954 + 963 WP_000756327.1 ParB/RepB/Spo0J family partition protein -
JKF55_RS25770 42188..42965 + 778 Protein_45 hypothetical protein -
JKF55_RS25775 43132..43275 + 144 WP_000270807.1 DUF1281 domain-containing protein -
JKF55_RS25780 43282..43641 + 360 WP_001385002.1 DUF1281 domain-containing protein -
JKF55_RS25785 43705..44016 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - eltB / eltA 1..75160 75160


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T186519 WP_001270417.1 NZ_CP068039:c38783-38496 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T186519 NZ_CP089551:1492342-1492620 [Pseudomonas sp. 7-41]
ATGATCAGAAGCTTTAAATGCGCCGAAACCCCAAAACTGTTTGAAACCGGTCGAACCCGACGTTGCTCTGCTGTCGTTTC
CGTGGTGGAACGACAGCTGAGCATGCTGGATGCGGCGGTTGAACTCGCTGACTTGCGCTCACCGCCCGGGAATAGGCTGG
AACCGTTATACGGTGACAGAGAGGGGCAACACAGTATCCGAATCAATGGTCAATTCCGGGTCTGTTTCATTTGGGGGCCT
CACGGCCCTGAAAATGTTGAAATTGTGGATTACCACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT186519 WP_001132895.1 NZ_CP068039:c39031-38780 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT186519 NZ_CP089551:1492629-1492934 [Pseudomonas sp. 7-41]
ATGTTTAAGAATGGCATGCGACCTGTACATCCTGGGGAAATACTCAAGGAAGAGTACCTGGAACCGCTGGGCCTGACCGC
AGCCGCTCTCGCAAGGGCGCTGCATGTTTCCGCCCCTACGGTGAATGACATTGTATTGATGCGTCGGAGCATCAGTGCGG
ACGTTGCATTACGCCTGGCAGTCGCGTTGGAGACATCTGCACAGTTCTGGCTGAACCTGCAGACCGCCTATGATCTGCGC
ACTGCGGAAATTGCCAAAGGCGCCCAGATTTACAAAGAAGTCGAGAGAGTTGCGCATTGCGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References