Detailed information of TA system TA186518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 17292..17839
• Replicon: plasmid pU90-4
• Accession: NZ_CP068039
• Organism: Escherichia coli strain U90

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: JKF55_RS25625
• Protein ID: WP_000969996.1
• Coordinates: 17558..17839 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2I6FMZ7
• Locus tag: JKF55_RS25620
• Protein ID: WP_000079930.1
• Coordinates: 17292..17561 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	13010..13071	-	62	NuclAT_0	-	-
-	13010..13071	-	62	NuclAT_0	-	-
-	13010..13071	-	62	NuclAT_0	-	-
-	13010..13071	-	62	NuclAT_0	-	-
JKF55_RS25600	14376..14516	+	141	WP_064717426.1	type I toxin-antitoxin system Hok family toxin	-
JKF55_RS25605	14796..15053	+	258	WP_000083834.1	replication regulatory protein RepA	-
JKF55_RS25610	15287..15361	+	75	WP_032319616.1	RepA leader peptide Tap	-
JKF55_RS25615	15354..16376	+	1023	WP_000840938.1	replication initiation protein	-
JKF55_RS25620	17292..17561	+	270	WP_000079930.1	hypothetical protein	Antitoxin
JKF55_RS25625	17558..17839	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JKF55_RS25630	17885..18614	+	730	Protein_17	3'-5' exonuclease	-
JKF55_RS25635	18684..18929	-	246	Protein_18	DDE-type integrase/transposase/recombinase	-
JKF55_RS25640	18946..20022	-	1077	WP_039022834.1	IS110 family transposase	-
JKF55_RS25645	20304..21163	-	860	Protein_20	IS3 family transposase	-
JKF55_RS25650	21277..21673	-	397	Protein_21	ATP-binding protein	-
JKF55_RS25655	22021..22395	-	375	WP_015675362.1	heat-labile enterotoxin LT subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltB / eltA	1..75160	75160
	-	inside	IScluster/Tn	-	eltB / eltA	10237..31783	21546

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T186518 WP_000969996.1 NZ_CP068039:17558-17839 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T186518 NZ_CP089551:c871801-871313 [Pseudomonas sp. 7-41]
GTGGAGCTAAAAGCCCCAGAAAGGCTGAACGATGAACACTCCCTGGATCAGTTCGATTCGGGTGAACAGTCGATCAACGA
TTACCTGCACAGGCAGGCGCGTAAGGCTCAAGCGGCCAAACAGGCCGTTGTTTACGTCGTCTGTCGCAAGGACACCGCTA
TTGTCATGGGTTACTACACCCTGTCGAGCGGTTCCATCGCACGCGAGAGCGTCGTTCCGAAAAGCCATCAACGCAACTCA
CCTAGCGTGCATCCAGTGACACTGCTGGGGCGCATGGGGATTACTCAAGAAGCTCAAGGCCAGGGTTATGCCATCGACCT
GCTGCAGGATGCAGTTGAACGGGCAATCAGCGCCTCCAATACGATTGGGTCGAGCGCAATGATTGTTCACCCGCTGAACG
AACGCCTGTCGAGTTTCTATACCAAGTACGCAGGATTTATACCGTGTCCGGGACTTTCGCCGATTACGTTGATGTTGCCG
CTTCGGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2055

>AT186518 WP_000079930.1 NZ_CP068039:17292-17561 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT186518 NZ_CP089551:c872085-871792 [Pseudomonas sp. 7-41]
ATGACTGCAATCATTGAAAGCATTGAAAAATCCAAGCCCGTTCCAATCAACATGAGAGCGGACGAAAAAAAACGGAACCT
GATCGATTTGGCAGCGGCAATGTCCGGTCGCGATCGAACCAGCTTTATCTTGGAGGCTGCTTGTCAAAAAGCAGAAGAGG
TCATCCTGGAAAAGCGCCTGTTCCTTCTCGACGACGTTGCATTTGACGCATTTGAACAGGCCATGGACGCCAACCCGATC
CGAGGTAACAAGTGCCTACAACAACTTCTAGAAAGACCCAAACGGTGGAGCTAA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2I6FMZ7