Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4835625..4836425 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag JKF55_RS23480 Protein ID WP_000342449.1
Coordinates 4835625..4836152 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag JKF55_RS23485 Protein ID WP_001277108.1
Coordinates 4836159..4836425 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS23455 4830700..4831467 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
JKF55_RS23460 4831464..4832741 - 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
JKF55_RS23465 4832738..4833664 - 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JKF55_RS23470 4833712..4834821 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JKF55_RS23475 4835245..4835628 + 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
JKF55_RS23480 4835625..4836152 - 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
JKF55_RS23485 4836159..4836425 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
JKF55_RS23490 4836575..4837678 - 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JKF55_RS23495 4837950..4838804 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JKF55_RS23500 4839049..4840107 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
JKF55_RS23505 4840100..4840768 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T186516 WP_000342449.1 NZ_CP068035:c4836152-4835625 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T186516 NZ_CP089536:c4763232-4762858 [Streptomyces sp. HNM0561]
ATGCACTACCTCACGCTGCCCGAGCTGCTGAACCTCGCCGAGCGCCTCGGGGAGAGCGAAGTGCGCGACTACGGGTTGCT
GGAGTCCGCGCTGGCCCGTCCGCAGGCGAGTGTGTTCGGGCAGGACGCCTACCCGGACGTGTGGCAGAAGGCCGCGGCGC
TGATGGAGTCGTCGGCTCGCAACCACGCCTTGGTGGACGGCAACAAGCGGCTCTCCTGGTATGCGACCTGGGTCTTCCTC
CACATCAATGGGCATCCGCTGCACGCGGACTTCGACGTCGACGAGGCCGAGCGCTTCGTACTGAGCGTCTGCCAGGGCGA
GCTGGACGTGGCGAAGATCGCGGAGGGGCTGACCCGGTTCACGGAAAGAATGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT186516 WP_001277108.1 NZ_CP068035:c4836425-4836159 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT186516 NZ_CP089536:c4763429-4763232 [Streptomyces sp. HNM0561]
ATGGCCGCGCTGAATCTACGTTTTACGGACGAAGAGCTCGACGCCTTGCGCAAGCGCGCGGAGGCGGAGGGCCGGAGCAT
GCAGTCGTTCGCCCACGACGCGGTGATGGCGGCGATCAATGAGCACTCCCGGCGGTTCGACGAGGCCGCCGACCATGTGT
TGCGGGTGAGCGAAGAGCTCAACCGGAGGCTCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References