Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4175596..4176250 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JKF55_RS20200 Protein ID WP_000244777.1
Coordinates 4175596..4176003 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JKF55_RS20205 Protein ID WP_000354046.1
Coordinates 4175984..4176250 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS20180 4171553..4173286 - 1734 WP_063103078.1 single-stranded-DNA-specific exonuclease RecJ -
JKF55_RS20185 4173292..4174002 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JKF55_RS20190 4174027..4174923 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JKF55_RS20195 4175035..4175556 + 522 WP_001055874.1 flavodoxin FldB -
JKF55_RS20200 4175596..4176003 - 408 WP_000244777.1 toxin CptA Toxin
JKF55_RS20205 4175984..4176250 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JKF55_RS20210 4176493..4177473 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JKF55_RS20215 4177550..4178209 - 660 WP_000250274.1 hemolysin III family protein -
JKF55_RS20220 4178373..4178684 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JKF55_RS20225 4178729..4180162 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
JKF55_RS20230 4180219..4180962 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T186512 WP_000244777.1 NZ_CP068035:c4176003-4175596 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186512 NZ_CP089532:c650418-649930 [Pseudomonas veronii]
GTGGAGCTAAAAGCCCCAGAAAGGCTGAACGATGAACACTCCCTGGATCAGTTCGATTCGGGTGAACAGTCGATCAACGA
TTACCTGCACAGGCAGGCGCGTAAGGCTCAAGCGGCCAAACAGGCCGTTGTTTACGTCGTCTGTCGCAAGGACACCGCTA
TCGTCATGGGTTACTACACCCTGTCGAGCGGTTCCATCGCACGCGAGAGCGTCGTTCCGAAAAGCCATCAACGCAACTCA
CCTAGCGTGCATCCAGTGACACTGCTGGGGCGCATGGGGATTACTCAAGAAGCTCAAGGCCAGGGTTATGCCATCGACCT
GCTGCAGGATGCAGTTGAACGGGCAATCAGCGCCTCCAATACGATTGGGTCGAGCGCAATGATTGTTCACCCGCTGAACG
AACGCCTGTCGAGTTTCTATACCAAGTACGCAGGATTTATACCGTGTCCGGGACTTTCGCCGATTACGTTGATGTTGCCG
CTTCGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186512 WP_000354046.1 NZ_CP068035:c4176250-4175984 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186512 NZ_CP089532:c650702-650409 [Pseudomonas veronii]
ATGACTGCAATCATTGAAAGCATTGAAAAATCCAAGCCCGTTCCAATCAACATGAGAGCGGACGAAAAAAAACGGAACCT
GATCGATTTGGCAGCGGCAATGTCCGGTCGCGATCGAACCAGCTTTATCTTGGAGGCTGCTTGTCAAAAAGCAGAAGAGG
TCATCCTGGAAAAGCGCCTGTTCCTTCTCGACGACGTTGCATTTGACGCATTTGAACAGGCCATGGACGCCAACCCGATC
CGAGGTAACAAGTGCCTACAACAACTTCTAGAAAGACCCAAACGGTGGAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References