Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4017972..4018555 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag JKF55_RS19440 Protein ID WP_000254738.1
Coordinates 4017972..4018307 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JKF55_RS19445 Protein ID WP_000581937.1
Coordinates 4018307..4018555 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS19425 4013859..4015157 - 1299 WP_000036723.1 phosphopyruvate hydratase -
JKF55_RS19430 4015245..4016882 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JKF55_RS19435 4017110..4017901 - 792 WP_063102844.1 nucleoside triphosphate pyrophosphohydrolase -
JKF55_RS19440 4017972..4018307 - 336 WP_000254738.1 endoribonuclease MazF Toxin
JKF55_RS19445 4018307..4018555 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JKF55_RS19450 4018633..4020867 - 2235 WP_000226815.1 GTP pyrophosphokinase -
JKF55_RS19455 4020915..4022216 - 1302 WP_000046812.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T186511 WP_000254738.1 NZ_CP068035:c4018307-4017972 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T186511 NZ_CP089532:363326-363607 [Pseudomonas veronii]
ATTGTCGCCCTGATCGAGATCATTGATTACATCGAGCAGTACAACCCGGTTGCCGCTGTCTCGCTGCACCAAACTATTGT
GGTTGCCGCCGAGGGGTTGTCCCTGATGCCATACGGATTCAAGCACGGTCGCTTGTCTGGTACTCGGGAAATGGTTGTTC
ATCCAAACTACCTGGTGGTCTATCGGGTGACGGAGCACGTTGAGATAGTGATGGTGTTACACACTCGCCAGGAATATCCC
AGGGATGTGATCGGGCCGCACCACTCGACCTCACGCCAGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT186511 WP_000581937.1 NZ_CP068035:c4018555-4018307 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT186511 NZ_CP089532:363120-363311 [Pseudomonas veronii]
ATGAACATTCCACGTTCTCCCATCGTTTCGGAGTTTGAAACAGAAGAGCAAGCTGCCAGCTACGACCGTTGGTTTCGTGC
CAAGGTGCAGGCTTCAATTGACGATCCTCGCCCTGGTATTCCGCACGATCAGGCGATGGCTGAAGTCGAGAAGGTGCTGG
AAGAGAGGCGCAAGGCTCGTGCTTCTCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References