Detailed information of TA system TA186509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3666887..3667512
• Replicon: chromosome
• Accession: NZ_CP068035
• Organism: Escherichia coli strain U90

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JKF55_RS17615
• Protein ID: WP_000911330.1
• Coordinates: 3666887..3667285 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JKF55_RS17620
• Protein ID: WP_000450524.1
• Coordinates: 3667285..3667512 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKF55_RS17595	3662765..3662965	+	201	WP_000383836.1	YpfN family protein	-
JKF55_RS17600	3663075..3663773	-	699	WP_000679812.1	esterase	-
JKF55_RS17605	3663847..3665862	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
JKF55_RS17610	3665877..3666740	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
JKF55_RS17615	3666887..3667285	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JKF55_RS17620	3667285..3667512	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JKF55_RS17625	3667666..3668379	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JKF55_RS17630	3668592..3669626	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
JKF55_RS17635	3669643..3670521	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JKF55_RS17640	3670667..3671239	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JKF55_RS17645	3671239..3671709	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T186509 WP_000911330.1 NZ_CP068035:c3667285-3666887 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T186509 NZ_CP089530:c1298002-1297256 [Bacillus amyloliquefaciens]
ATGCTTATGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT186509 WP_000450524.1 NZ_CP068035:c3667512-3667285 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT186509 NZ_CP089530:c1297255-1297085 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33