Detailed information of TA system TA186497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2688541..2689067
• Replicon: chromosome
• Accession: NZ_CP068035
• Organism: Escherichia coli strain U90

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JKF55_RS12925
• Protein ID: WP_000323025.1
• Coordinates: 2688780..2689067 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JKF55_RS12920
• Protein ID: WP_000534858.1
• Coordinates: 2688541..2688780 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKF55_RS12895	2683903..2684259	+	357	WP_001310834.1	class I SAM-dependent methyltransferase	-
JKF55_RS12900	2684870..2685157	+	288	Protein_2508	hypothetical protein	-
JKF55_RS12905	2685926..2687239	+	1314	WP_000589005.1	ISNCY family transposase	-
JKF55_RS12910	2687676..2688008	-	333	WP_001301033.1	protein FlxA	-
JKF55_RS12915	2688211..2688516	-	306	WP_001326990.1	hypothetical protein	-
JKF55_RS12920	2688541..2688780	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JKF55_RS12925	2688780..2689067	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JKF55_RS12930	2689139..2689294	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
JKF55_RS12935	2689511..2689762	+	252	WP_000980994.1	hypothetical protein	-
JKF55_RS12940	2689829..2690107	+	279	WP_012304870.1	hypothetical protein	-
JKF55_RS12945	2690109..2691158	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
JKF55_RS12950	2691172..2691924	+	753	WP_001047135.1	antitermination protein	-
JKF55_RS12955	2692202..2692291	-	90	WP_120795389.1	hypothetical protein	-
JKF55_RS12960	2692346..2692558	-	213	WP_000087756.1	cold shock-like protein CspF	-
JKF55_RS12965	2692859..2693074	+	216	WP_000066484.1	cold shock-like protein CspB	-
JKF55_RS12970	2693828..2694043	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2671388..2724380	52992

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T186497 WP_000323025.1 NZ_CP068035:2688780-2689067 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T186497 NZ_CP089509:c5177050-5176667 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT186497 WP_000534858.1 NZ_CP068035:2688541-2688780 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT186497 NZ_CP089509:c5177292-5177050 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8