Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 628195..628790 Replicon chromosome
Accession NZ_CP068035
Organism Escherichia coli strain U90

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag JKF55_RS03000 Protein ID WP_000239579.1
Coordinates 628440..628790 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag JKF55_RS02995 Protein ID WP_001223208.1
Coordinates 628195..628446 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF55_RS02940 623573..624109 - 537 WP_077249062.1 DNA-binding protein -
JKF55_RS02945 624139..624438 - 300 WP_064986856.1 ead/Ea22-like family protein -
JKF55_RS02950 624435..624680 - 246 WP_000153674.1 winged helix-turn-helix domain-containing protein -
JKF55_RS02955 624677..624880 - 204 WP_001560998.1 hypothetical protein -
JKF55_RS02960 624955..625080 - 126 WP_040091107.1 hypothetical protein -
JKF55_RS02965 625077..625319 - 243 WP_000357024.1 DUF4754 family protein -
JKF55_RS02970 625331..625609 - 279 WP_040091109.1 hypothetical protein -
JKF55_RS02975 625620..625970 - 351 WP_064986855.1 DUF4761 family protein -
JKF55_RS02980 626008..626433 - 426 WP_001158283.1 regulatory protein -
JKF55_RS02985 626542..626841 + 300 WP_000108576.1 helix-turn-helix transcriptional regulator -
JKF55_RS02990 626908..627903 + 996 WP_001519231.1 tyrosine-type recombinase/integrase -
JKF55_RS02995 628195..628446 + 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JKF55_RS03000 628440..628790 + 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
JKF55_RS03005 628870..629400 - 531 WP_000055072.1 inorganic diphosphatase -
JKF55_RS03010 629710..630666 + 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JKF55_RS03015 630976..632478 + 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
JKF55_RS03020 632492..633514 + 1023 WP_001339490.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 593124..642598 49474


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T186489 WP_000239579.1 NZ_CP068035:628440-628790 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T186489 NZ_CP089509:813175-813585 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT186489 WP_001223208.1 NZ_CP068035:628195-628446 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT186489 NZ_CP089509:812928-813194 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References