Detailed information of TA system TA186487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5679630..5680149
• Replicon: chromosome
• Accession: NZ_CP068034
• Organism: Pseudomonas syringae strain BIM B-268

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JJQ97_RS24330
• Protein ID: WP_201418806.1
• Coordinates: 5679630..5679920 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6UWW5
• Locus tag: JJQ97_RS24335
• Protein ID: WP_002551444.1
• Coordinates: 5679910..5680149 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJQ97_RS24295	5674693..5675385	+	693	WP_201418801.1	DUF4225 domain-containing protein	-
JJQ97_RS24300	5675900..5676223	+	324	WP_201418802.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JJQ97_RS24305	5676199..5676564	+	366	WP_201420373.1	putative addiction module antidote protein	-
JJQ97_RS24310	5676657..5677301	-	645	WP_201418803.1	histidine phosphatase family protein	-
JJQ97_RS24315	5677401..5677742	-	342	WP_046235977.1	hypothetical protein	-
JJQ97_RS24320	5677932..5678354	+	423	WP_201418804.1	DUF1493 family protein	-
JJQ97_RS24325	5678663..5679400	+	738	WP_201418805.1	hypothetical protein	-
JJQ97_RS24330	5679630..5679920	-	291	WP_201418806.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJQ97_RS24335	5679910..5680149	-	240	WP_002551444.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JJQ97_RS24340	5680311..5680784	-	474	WP_201420374.1	GNAT family N-acetyltransferase	-
JJQ97_RS24345	5680880..5681545	-	666	Protein_4790	transcriptional regulator	-
JJQ97_RS24350	5681520..5682089	-	570	WP_201418807.1	GNAT family N-acetyltransferase	-
JJQ97_RS24355	5682250..5682744	+	495	WP_201420375.1	hypothetical protein	-
JJQ97_RS24360	5682782..5684056	-	1275	WP_201418808.1	OprD family porin	-
JJQ97_RS24365	5684278..5684484	-	207	WP_201420376.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11272.18 Da
• Isoelectric Point: 10.3662

>T186487 WP_201418806.1 NZ_CP068034:c5679920-5679630 [Pseudomonas syringae]
MTYNLEFDARALKDWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDKEVVVFVVAVDK
REREEVYRKAADRLGR

Nucleotide

• Length: 291 bp

>T186487 NZ_CP089509:355975-356322 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8955.34 Da
• Isoelectric Point: 4.6017

>AT186487 WP_002551444.1 NZ_CP068034:c5680149-5679910 [Pseudomonas syringae]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT186487 NZ_CP089509:356322-356621 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATGGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8M1G9