Detailed information of TA system TA186483

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4591810..4592652
• Replicon: chromosome
• Accession: NZ_CP068033
• Organism: Klebsiella pneumoniae strain LYS105A

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JKA77_RS22350
• Protein ID: WP_040246536.1
• Coordinates: 4591810..4592187 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JKA77_RS22355
• Protein ID: WP_077256055.1
• Coordinates: 4592239..4592652 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JKA77_RS22320	4587608..4587796	-	189	Protein_4372	transposase	-
JKA77_RS22325	4587813..4588924	-	1112	Protein_4373	IS3 family transposase	-
JKA77_RS22330	4589239..4590036	-	798	WP_040246544.1	helix-turn-helix transcriptional regulator	-
JKA77_RS22335	4590180..4591025	-	846	WP_040246542.1	DUF4942 domain-containing protein	-
JKA77_RS22340	4591107..4591292	-	186	WP_040246540.1	DUF957 domain-containing protein	-
JKA77_RS22345	4591322..4591813	-	492	WP_040246538.1	hypothetical protein	-
JKA77_RS22350	4591810..4592187	-	378	WP_040246536.1	TA system toxin CbtA family protein	Toxin
JKA77_RS22355	4592239..4592652	-	414	WP_077256055.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JKA77_RS22360	4592621..4592842	-	222	WP_040246532.1	DUF987 domain-containing protein	-
JKA77_RS22365	4592863..4593342	-	480	WP_040246529.1	DNA repair protein RadC	-
JKA77_RS22370	4593354..4593824	-	471	WP_040246527.1	antirestriction protein	-
JKA77_RS22375	4594064..4594255	-	192	WP_100097506.1	DUF905 family protein	-
JKA77_RS22380	4594311..4594532	-	222	WP_040246525.1	hypothetical protein	-
JKA77_RS22385	4594606..4595076	-	471	WP_040246523.1	hypothetical protein	-
JKA77_RS22390	4595127..4595438	-	312	Protein_4386	hypothetical protein	-
JKA77_RS22395	4595767..4596378	-	612	Protein_4387	IS256 family transposase	-
JKA77_RS22400	4596552..4597517	-	966	WP_040246521.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4577808..4619793	41985
	-	flank	IS/Tn	-	-	4595659..4596378	719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14021.73 Da
• Isoelectric Point: 5.5540

>T186483 WP_040246536.1 NZ_CP068033:c4592187-4591810 [Klebsiella pneumoniae]
MQTQPLSSTQEASPRPSQVDIWQQLLSHLLDRHYGLTLNDTPFGHDGVIQEHIDAGISLCDAVNVIVEKYDLVRTDRPGF
SAETQSPLISSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T186483 NZ_CP089448:4524509-4524727 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14964.07 Da
• Isoelectric Point: 6.8319

>AT186483 WP_077256055.1 NZ_CP068033:c4592652-4592239 [Klebsiella pneumoniae]
MSSCAVSRSINVNLRKAKLSNEIPAVNHDIAEPWWGLKPTVTPCFGARLVQEGNQVHYLADRASITGQFSEADLRHLDQA
FPVLLKQLELMLVSGELNPHHQHGVTLYAKGLTCEADSLGSHGYIYIAIYPTPAATA

Nucleotide

• Length: 414 bp

>AT186483 NZ_CP089448:4524108-4524482 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG