Detailed information of TA system TA186469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1875602..1876079
• Replicon: chromosome
• Accession: NZ_CP068031
• Organism: Staphylococcus saccharolyticus strain DVP5-16-4677

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: -
• Locus tag: DMB75_RS09030
• Protein ID: WP_130547011.1
• Coordinates: 1875891..1876079 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: -
• Locus tag: DMB75_RS09025
• Protein ID: WP_130547012.1
• Coordinates: 1875602..1875859 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB75_RS08990	1870980..1871522	-	543	WP_130547018.1	nitroreductase	-
DMB75_RS08995	1871601..1872317	-	717	WP_130547017.1	type I 3-dehydroquinate dehydratase	-
DMB75_RS09000	1872482..1872904	+	423	WP_130547016.1	organic hydroperoxide resistance protein	-
DMB75_RS09005	1873306..1873812	+	507	WP_130547015.1	GNAT family N-acetyltransferase	-
DMB75_RS09010	1873919..1874503	-	585	WP_130547014.1	phosphoglycerate mutase family protein	-
DMB75_RS09015	1874724..1874960	+	237	WP_115313759.1	hypothetical protein	-
DMB75_RS09020	1874952..1875155	-	204	WP_130547013.1	hypothetical protein	-
DMB75_RS09025	1875602..1875859	+	258	WP_130547012.1	hypothetical protein	Antitoxin
DMB75_RS09030	1875891..1876079	+	189	WP_130547011.1	hypothetical protein	Toxin
DMB75_RS09035	1876284..1876862	+	579	WP_130547010.1	hypothetical protein	-
DMB75_RS09040	1876922..1877155	-	234	WP_130547009.1	hypothetical protein	-
DMB75_RS09045	1877554..1878300	+	747	WP_130547008.1	poly-gamma-glutamate hydrolase family protein	-
DMB75_RS09050	1878404..1878688	+	285	WP_130547007.1	hypothetical protein	-
DMB75_RS09055	1879627..1879824	+	198	WP_130547006.1	hypothetical protein	-
DMB75_RS09060	1879863..1880063	-	201	WP_130547005.1	cold-shock protein	-
DMB75_RS09065	1880798..1880869	+	72	WP_126517390.1	epsilon family phenol-soluble modulin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7210.93 Da
• Isoelectric Point: 8.5299

>T186469 WP_130547011.1 NZ_CP068031:1875891-1876079 [Staphylococcus saccharolyticus]
MSLHFYILLWLAILFIIAGTILLITMLNTKKEERKESYLGFTVIFLIFGITILIYTIIFGIL

Nucleotide

• Length: 189 bp

>T186469 NZ_CP089445:c4585135-4584608 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.03 Da
• Isoelectric Point: 3.7781

>AT186469 WP_130547012.1 NZ_CP068031:1875602-1875859 [Staphylococcus saccharolyticus]
MKLIIINVINVILTILFMAFNVLITYNENINNNLWLVPGLIIFGITLLTSLTIAIVYTDLFSEILFFINIVLALYYIYPI
IYEFI

Nucleotide

• Length: 258 bp

>AT186469 NZ_CP089445:c4585408-4585142 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA