Detailed information of TA system TA186467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 824423..824952
• Replicon: chromosome
• Accession: NZ_CP068031
• Organism: Staphylococcus saccharolyticus strain DVP5-16-4677

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: DMB75_RS03890
• Protein ID: WP_130548321.1
• Coordinates: 824590..824952 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: DMB75_RS03885
• Protein ID: WP_115312958.1
• Coordinates: 824423..824593 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB75_RS03860	820237..820715	+	479	Protein_757	PH domain-containing protein	-
DMB75_RS03865	820708..822206	+	1499	Protein_758	PH domain-containing protein	-
DMB75_RS03870	822193..822675	+	483	WP_130548324.1	PH domain-containing protein	-
DMB75_RS03875	822758..823111	+	354	WP_130548323.1	holo-ACP synthase	-
DMB75_RS03880	823188..824336	+	1149	WP_130548322.1	alanine racemase	-
DMB75_RS03885	824423..824593	+	171	WP_115312958.1	antitoxin MazE	Antitoxin
DMB75_RS03890	824590..824952	+	363	WP_130548321.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
DMB75_RS03895	825303..826305	+	1003	Protein_764	PP2C family protein-serine/threonine phosphatase	-
DMB75_RS03900	826407..826733	+	327	WP_130548319.1	anti-sigma factor antagonist	-
DMB75_RS03905	826735..827214	+	480	WP_115312962.1	anti-sigma B factor RsbW	-
DMB75_RS03910	827189..827959	+	771	WP_130548318.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13533.68 Da
• Isoelectric Point: 10.0249

>T186467 WP_130548321.1 NZ_CP068031:824590-824952 [Staphylococcus saccharolyticus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSESKMKEVDNALDISLGLHNFDQQKS

Nucleotide

• Length: 363 bp

>T186467 NZ_CP089445:c4232376-4232062 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6257.82 Da
• Isoelectric Point: 3.6326

>AT186467 WP_115312958.1 NZ_CP068031:824423-824593 [Staphylococcus saccharolyticus]
MLSFNQTRTNSLEQSLKEGYSQMADLNLSLATEAFPIECEACDCNETYLTSNSQSE

Nucleotide

• Length: 171 bp

>AT186467 NZ_CP089445:c4232065-4231649 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA