Detailed information of TA system TA186466

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 813192..813691
• Replicon: chromosome
• Accession: NZ_CP068031
• Organism: Staphylococcus saccharolyticus strain DVP5-16-4677

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: DMB75_RS03825
• Protein ID: WP_130548329.1
• Coordinates: 813512..813691 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: -
• Locus tag: DMB75_RS03820
• Protein ID: WP_130548330.1
• Coordinates: 813192..813485 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DMB75_RS03795	808318..809106	+	789	WP_130548334.1	hydroxyethylthiazole kinase	-
DMB75_RS03800	809110..809751	+	642	WP_130548333.1	thiamine phosphate synthase	-
DMB75_RS03805	809840..810712	+	873	WP_130548332.1	membrane protein insertase YidC	-
DMB75_RS03810	810713..811418	-	706	Protein_747	HD domain-containing protein	-
DMB75_RS03815	811523..813007	-	1485	WP_130548331.1	cardiolipin synthase	-
DMB75_RS03820	813192..813485	+	294	WP_130548330.1	copper-sensing transcriptional repressor CsoR	Antitoxin
DMB75_RS03825	813512..813691	+	180	WP_130548329.1	heavy-metal-associated domain-containing protein	Toxin
DMB75_RS03830	813831..813968	+	138	WP_115312949.1	hypothetical protein	-
DMB75_RS03835	814166..815377	-	1212	WP_130548328.1	rod shape-determining protein RodA	-
DMB75_RS03840	815548..815712	+	165	WP_165418024.1	hypothetical protein	-
DMB75_RS03845	815784..816854	+	1071	WP_130548327.1	D-alanine--D-alanine ligase	-
DMB75_RS03850	816870..818225	+	1356	WP_130548326.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6453.33 Da
• Isoelectric Point: 7.2000

>T186466 WP_130548329.1 NZ_CP068031:813512-813691 [Staphylococcus saccharolyticus]
IIHVSGIETGEQEAQLTKLLSKMIGAQSVTANRISGIRTPKNLNSIEKEIYDSGYHVVN

Nucleotide

• Length: 180 bp

>T186466 NZ_CP089445:4056868-4057245 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11003.51 Da
• Isoelectric Point: 5.5723

>AT186466 WP_130548330.1 NZ_CP068031:813192-813485 [Staphylococcus saccharolyticus]
MTENDTAHHSEQTKANLKSRLNRIEGQVCAINRMIEDDVYCDDVLTQIKATRSALNSVATKLLDHHMKSCIMDKVNNGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT186466 NZ_CP089445:4056411-4056779 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA