Detailed information of TA system TA186462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3651305..3653019
• Replicon: chromosome
• Accession: NZ_CP068023
• Organism: Pseudoalteromonas sp. GCY

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JJQ94_RS21805
• Protein ID: WP_099031722.1
• Coordinates: 3651305..3651955 (+)
• Length: 217 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: JJQ94_RS21810
• Protein ID: WP_099031721.1
• Coordinates: 3651955..3653019 (+)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJQ94_RS21785	3646812..3647051	+	240	WP_045964924.1	hypothetical protein	-
JJQ94_RS21790	3647086..3649158	+	2073	Protein_3177	SAM-dependent DNA methyltransferase	-
JJQ94_RS21795	3649696..3649935	+	240	Protein_3178	hypothetical protein	-
JJQ94_RS21800	3649935..3651302	+	1368	WP_099031723.1	restriction endonuclease subunit S	-
JJQ94_RS21805	3651305..3651955	+	651	WP_099031722.1	DUF4433 domain-containing protein	Toxin
JJQ94_RS21810	3651955..3653019	+	1065	WP_099031721.1	macro domain-containing protein	Antitoxin
JJQ94_RS21815	3653057..3656218	+	3162	WP_099031720.1	HsdR family type I site-specific deoxyribonuclease	-
JJQ94_RS21820	3656218..3656964	+	747	WP_099031719.1	M48 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-128)

Sequences

Toxin

Protein

• Length: 217 a.a.
• Molecular weight: 25060.71 Da
• Isoelectric Point: 7.8434

>T186462 WP_099031722.1 NZ_CP068023:3651305-3651955 [Pseudoalteromonas sp. GCY]
VVYNYSQTLNPQKALIWRIVHRNNIPWILDNGLYCGNSQVQSPNWEAIGSSELIQKRATHPVPIAPFGSLNDYVPFYFTP
FSPMLLNIKSGRGVIQRANEDIVILVSSLHKVEQLNLEFVYTDMHAYYLWAKFYSDLKHLGAVDWPILQARDFARDQNDP
AKFERYQAEALIKQHCPINALLGVVCYNAQVEQQLKQHIQQRNMNLDVIVRPGWYF

Nucleotide

• Length: 651 bp

>T186462 NZ_CP089445:c2943686-2943432 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 40032.01 Da
• Isoelectric Point: 6.1668

>AT186462 WP_099031721.1 NZ_CP068023:3651955-3653019 [Pseudoalteromonas sp. GCY]
MITYTQGNLLEANADALVNTVNTVGVMGKGIALMFKERFPKNMAEYAAACKAKRVNTGQMFVTETGELMGPRWIVNFPTK
QHWRAKSKMEWIQDGLIDLRHFIENNNVGSIAIPPLGAGQGGLSWPDVKLEIEKALSDIENTNIIVFEPTSKYQNVAKKA
GVKKLTPARALVAELVRRYWILGMECSLLEIQKLAWFLQRTIERSGLKNELKLNFEANYYGPYANNLEHLLNALDGSYLK
SDKRIPDCDPLDVIWFNENERQKVSTYLSTEAKEYLPALEEATQVINGFESPFGLELLSTVDWLITKQGCDKTVESIKEG
IANWPTGDKWALRKLTLFGDREIQFALTNLENLH

Nucleotide

• Length: 1065 bp

>AT186462 NZ_CP089445:c2943934-2943683 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA