Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5519754..5520379 Replicon chromosome
Accession NZ_CP068015
Organism Klebsiella pneumoniae strain 2016_49

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag JKF56_RS26805 Protein ID WP_012737091.1
Coordinates 5519754..5520137 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag JKF56_RS26810 Protein ID WP_004150355.1
Coordinates 5520137..5520379 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF56_RS26790 (5517120) 5517120..5518022 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
JKF56_RS26795 (5518019) 5518019..5518654 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
JKF56_RS26800 (5518651) 5518651..5519580 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
JKF56_RS26805 (5519754) 5519754..5520137 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
JKF56_RS26810 (5520137) 5520137..5520379 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
JKF56_RS26815 (5520584) 5520584..5521501 + 918 WP_012737090.1 alpha/beta hydrolase -
JKF56_RS26820 (5521515) 5521515..5522456 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
JKF56_RS26825 (5522501) 5522501..5522938 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
JKF56_RS26830 (5522935) 5522935..5523795 - 861 WP_004146232.1 virulence factor BrkB family protein -
JKF56_RS26835 (5523789) 5523789..5524388 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T186431 WP_012737091.1 NZ_CP068015:c5520137-5519754 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T186431 NZ_CP089437:c4951304-4950930 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGCGGTAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGTACTCGCGTTGCGCTAGGCGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAGCATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
AAATACCAAGGATTTTGCAGGCATATCAGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT186431 WP_004150355.1 NZ_CP068015:c5520379-5520137 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT186431 NZ_CP089437:c4951546-4951304 [Citrobacter portucalensis]
ATGATGGTTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAATCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACAA
CAATTTCACGCGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References