Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4881504..4882314 Replicon chromosome
Accession NZ_CP068015
Organism Klebsiella pneumoniae strain 2016_49

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag JKF56_RS23820 Protein ID WP_014908042.1
Coordinates 4881504..4882037 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag JKF56_RS23825 Protein ID WP_002887278.1
Coordinates 4882048..4882314 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF56_RS23815 (4880335) 4880335..4881456 + 1122 WP_012737335.1 cupin domain-containing protein -
JKF56_RS23820 (4881504) 4881504..4882037 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
JKF56_RS23825 (4882048) 4882048..4882314 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
JKF56_RS23830 (4882417) 4882417..4883850 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
JKF56_RS23835 (4883840) 4883840..4884523 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
JKF56_RS23840 (4884695) 4884695..4886080 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
JKF56_RS23845 (4886098) 4886098..4886442 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T186429 WP_014908042.1 NZ_CP068015:c4882037-4881504 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T186429 NZ_CP089437:4795338-4795655 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTCTGGCTAATGGTTTAGCTGGTGATGTTAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGTTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTCGCCCGTACACTGGATTCACAGGAGATCTTCTGCCATGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT186429 WP_002887278.1 NZ_CP068015:c4882314-4882048 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT186429 NZ_CP089437:4795648-4795926 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGCGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTAGCTCGCGCTAAGGGAATGGCGAACATTGCGGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACCCTTGCAGTG
ATGAAGGTACTCGGCATTGAATTGACACTTAAAAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References