Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4290234..4290853 Replicon chromosome
Accession NZ_CP068015
Organism Klebsiella pneumoniae strain 2016_49

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JKF56_RS20975 Protein ID WP_002892050.1
Coordinates 4290635..4290853 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JKF56_RS20970 Protein ID WP_002892066.1
Coordinates 4290234..4290608 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JKF56_RS20960 (4285386) 4285386..4286579 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JKF56_RS20965 (4286602) 4286602..4289748 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JKF56_RS20970 (4290234) 4290234..4290608 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JKF56_RS20975 (4290635) 4290635..4290853 + 219 WP_002892050.1 HHA domain-containing protein Toxin
JKF56_RS20980 (4291012) 4291012..4291578 + 567 WP_002892030.1 maltose O-acetyltransferase -
JKF56_RS20985 (4291550) 4291550..4291690 - 141 WP_004147370.1 hypothetical protein -
JKF56_RS20990 (4291711) 4291711..4292181 + 471 WP_002892026.1 YlaC family protein -
JKF56_RS20995 (4292156) 4292156..4293607 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
JKF56_RS21000 (4293708) 4293708..4294406 + 699 WP_002892021.1 GNAT family protein -
JKF56_RS21005 (4294403) 4294403..4294543 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JKF56_RS21010 (4294543) 4294543..4294806 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186428 WP_002892050.1 NZ_CP068015:4290635-4290853 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T186428 NZ_CP089437:4653335-4653712 [Citrobacter portucalensis]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT186428 WP_002892066.1 NZ_CP068015:4290234-4290608 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT186428 NZ_CP089437:4652919-4653245 [Citrobacter portucalensis]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References