Detailed information of TA system TA186410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4007226..4008040
• Replicon: chromosome
• Accession: NZ_CP067994
• Organism: Escherichia coli strain CV601

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: JJT18_RS19490
• Protein ID: WP_001054376.1
• Coordinates: 4007226..4007483 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: JJT18_RS19495
• Protein ID: WP_001309181.1
• Coordinates: 4007495..4008040 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJT18_RS19470	4002514..4003620	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
JJT18_RS19475	4003685..4004665	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JJT18_RS19480	4005248..4006488	-	1241	Protein_3805	DNA helicase	-
JJT18_RS19485	4006604..4006849	+	246	Protein_3806	GNAT family N-acetyltransferase	-
JJT18_RS19490	4007226..4007483	+	258	WP_001054376.1	hypothetical protein	Toxin
JJT18_RS19495	4007495..4008040	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
JJT18_RS19500	4008096..4008842	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
JJT18_RS19505	4009011..4009229	+	219	Protein_3810	hypothetical protein	-
JJT18_RS19510	4009267..4009383	+	117	Protein_3811	VOC family protein	-
JJT18_RS19515	4009628..4010749	+	1122	WP_000010829.1	M42 family metallopeptidase	-
JJT18_RS19520	4010746..4011024	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JJT18_RS19525	4011036..4012349	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	3998477..4016264	17787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T186410 WP_001054376.1 NZ_CP067994:4007226-4007483 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T186410 NZ_CP089427:4010561-4010779 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT186410 WP_001309181.1 NZ_CP067994:4007495-4008040 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT186410 NZ_CP089427:4010160-4010534 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8