Detailed information of TA system TA186405

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3579855..3580534
• Replicon: chromosome
• Accession: NZ_CP067994
• Organism: Escherichia coli strain CV601

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: JJT18_RS17420
• Protein ID: WP_000854672.1
• Coordinates: 3579855..3580196 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: JJT18_RS17425
• Protein ID: WP_000070395.1
• Coordinates: 3580217..3580534 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJT18_RS17395	3575441..3575533	+	93	Protein_3399	sigma factor-binding protein Crl	-
JJT18_RS17400	3575572..3576627	-	1056	WP_000749863.1	phosphoporin PhoE	-
JJT18_RS17405	3576915..3578018	+	1104	WP_001285288.1	glutamate 5-kinase	-
JJT18_RS17410	3578030..3579283	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
JJT18_RS17420	3579855..3580196	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
JJT18_RS17425	3580217..3580534	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JJT18_RS17430	3580553..3580774	-	222	WP_000691994.1	DUF987 domain-containing protein	-
JJT18_RS17435	3580783..3581259	-	477	WP_000811693.1	RadC family protein	-
JJT18_RS17440	3581275..3581733	-	459	WP_000211838.1	antirestriction protein	-
JJT18_RS17445	3581831..3582070	-	240	WP_000194654.1	DUF905 domain-containing protein	-
JJT18_RS17450	3582147..3582614	-	468	WP_001547765.1	hypothetical protein	-
JJT18_RS17455	3582637..3583080	-	444	WP_000824223.1	hypothetical protein	-
JJT18_RS17460	3583080..3583307	-	228	WP_001548158.1	hypothetical protein	-
JJT18_RS17465	3583303..3583494	-	192	Protein_3412	DeoR family transcriptional regulator	-
JJT18_RS17470	3583711..3584532	-	822	WP_000197389.1	DUF945 domain-containing protein	-
JJT18_RS17475	3584624..3585487	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3574735..3598498	23763

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T186405 WP_000854672.1 NZ_CP067994:c3580196-3579855 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T186405 NZ_CP089427:353125-353472 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT186405 WP_000070395.1 NZ_CP067994:c3580534-3580217 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT186405 NZ_CP089427:353472-353771 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684