Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 847912..848566 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JJT18_RS04090 Protein ID WP_000244777.1
Coordinates 848159..848566 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JJT18_RS04085 Protein ID WP_000354046.1
Coordinates 847912..848178 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJT18_RS04060 843081..843824 + 744 WP_000951964.1 SDR family oxidoreductase -
JJT18_RS04065 843881..845314 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JJT18_RS04070 845359..845670 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JJT18_RS04075 845834..846493 + 660 WP_000250274.1 hemolysin III family protein -
JJT18_RS04080 846689..847669 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JJT18_RS04085 847912..848178 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JJT18_RS04090 848159..848566 + 408 WP_000244777.1 toxin CptA Toxin
JJT18_RS04095 848606..849127 - 522 WP_001055874.1 flavodoxin FldB -
JJT18_RS04100 849239..850135 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JJT18_RS04105 850160..850870 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JJT18_RS04110 850876..852609 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T186387 WP_000244777.1 NZ_CP067994:848159-848566 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186387 NZ_CP089407:c4013271-4012984 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186387 WP_000354046.1 NZ_CP067994:847912-848178 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186387 NZ_CP089407:c4012997-4012695 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGCTGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References