Detailed information of TA system TA186378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 844131..845377
• Replicon: chromosome
• Accession: NZ_CP067992
• Organism: Streptococcus mitis strain S022-V7-A3

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: JJN14_RS04165
• Protein ID: WP_201059015.1
• Coordinates: 844607..845377 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: JJN14_RS04160
• Protein ID: WP_166731334.1
• Coordinates: 844131..844607 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN14_RS04150	839626..840768	+	1143	WP_201059013.1	hypothetical protein	-
JJN14_RS04155	840770..843811	+	3042	WP_201059014.1	DEAD/DEAH box helicase family protein	-
JJN14_RS04160	844131..844607	+	477	WP_166731334.1	helix-turn-helix domain-containing protein	Antitoxin
JJN14_RS04165	844607..845377	+	771	WP_201059015.1	zeta toxin family protein	Toxin
JJN14_RS04170	845732..846628	-	897	WP_201059016.1	tyrosine-type recombinase/integrase	-
JJN14_RS04175	846726..847442	+	717	WP_201059017.1	type 11 methyltransferase	-
JJN14_RS04180	847463..848452	-	990	WP_201059018.1	hypothetical protein	-
JJN14_RS04185	848778..849782	+	1005	WP_201059019.1	NADP-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	836056..850443	14387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29410.46 Da
• Isoelectric Point: 8.7888

>T186378 WP_201059015.1 NZ_CP067992:844607-845377 [Streptococcus mitis]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVKYTKDFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEYHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAATVLQELLFGEWSQVEKEML
KSGEERLRDLTNRNGC

Nucleotide

• Length: 771 bp

>T186378 NZ_CP089403:c2824974-2824756 [Klebsiella pasteurii]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCACCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18257.74 Da
• Isoelectric Point: 4.4188

>AT186378 WP_166731334.1 NZ_CP067992:844131-844607 [Streptococcus mitis]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYIGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT186378 NZ_CP089403:c2825375-2825001 [Klebsiella pasteurii]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCAACTTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG