Detailed information of TA system TA186373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2691563..2693151
• Replicon: chromosome
• Accession: NZ_CP067966
• Organism: Klebsiella quasipneumoniae strain ARLG-8054

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JJ669_RS13175
• Protein ID: WP_044523012.1
• Coordinates: 2691563..2692885 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: JJ669_RS13180
• Protein ID: WP_023289667.1
• Coordinates: 2692885..2693151 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJ669_RS13160 (2686607)	2686607..2689120	+	2514	WP_240247621.1	fimbria/pilus outer membrane usher protein	-
JJ669_RS13165 (2689108)	2689108..2690112	+	1005	WP_240247165.1	fimbrial protein	-
JJ669_RS13170 (2690254)	2690254..2691546	+	1293	WP_044523014.1	glycoside hydrolase family 10 protein	-
JJ669_RS13175 (2691563)	2691563..2692885	-	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
JJ669_RS13180 (2692885)	2692885..2693151	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JJ669_RS13185 (2693416)	2693416..2693742	-	327	WP_044523006.1	hydroxyisourate hydrolase	-
JJ669_RS13190 (2693739)	2693739..2694239	-	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
JJ669_RS13195 (2694217)	2694217..2695596	-	1380	WP_032452824.1	nucleobase:cation symporter-2 family protein	-
JJ669_RS13200 (2695942)	2695942..2697096	+	1155	WP_023289663.1	FAD-dependent urate hydroxylase HpxO	-
JJ669_RS13205 (2697077)	2697077..2698000	-	924	WP_240247166.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T186373 WP_044523012.1 NZ_CP067966:c2692885-2691563 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T186373 NZ_CP089395:c5827668-5827327 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT186373 WP_023289667.1 NZ_CP067966:c2693151-2692885 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT186373 NZ_CP089395:c5827317-5827018 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3