Detailed information of TA system TA186362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 73568..74193
• Replicon: plasmid p118
• Accession: NZ_CP067431
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JI426_RS28270
• Protein ID: WP_000911320.1
• Coordinates: 73568..73966 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2A2C6V4
• Locus tag: JI426_RS28275
• Protein ID: WP_000450527.1
• Coordinates: 73966..74193 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS28250 (69163)	69163..69660	+	498	WP_032211977.1	entry exclusion protein	-
JI426_RS28255 (69692)	69692..70423	+	732	WP_028985894.1	conjugal transfer complement resistance protein TraT	-
JI426_RS28260 (70495)	70495..71106	+	612	WP_000908442.1	hypothetical protein	-
JI426_RS28265 (71388)	71388..73559	+	2172	WP_249869019.1	type IV conjugative transfer system coupling protein TraD	-
JI426_RS28270 (73568)	73568..73966	-	399	WP_000911320.1	type II toxin-antitoxin system VapC family toxin	Toxin
JI426_RS28275 (73966)	73966..74193	-	228	WP_000450527.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..118340	118340

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14945.21 Da
• Isoelectric Point: 7.8605

>T186362 WP_000911320.1 NZ_CP067431:c73966-73568 [Escherichia coli O22:H8]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDATAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T186362 NZ_CP089385:c4985943-4985659 [Klebsiella variicola subsp. variicola]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGATGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8542.54 Da
• Isoelectric Point: 4.4013

>AT186362 WP_000450527.1 NZ_CP067431:c74193-73966 [Escherichia coli O22:H8]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSDDFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT186362 NZ_CP089385:c4986175-4985933 [Klebsiella variicola subsp. variicola]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A2A2C6V4