Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19560..20085 Replicon plasmid p118
Accession NZ_CP067431
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag JI426_RS27920 Protein ID WP_001159868.1
Coordinates 19780..20085 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag JI426_RS27915 Protein ID WP_000813634.1
Coordinates 19560..19778 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS27900 (14716) 14716..15615 + 900 WP_000963206.1 nucleotide-binding protein -
JI426_RS27905 (15665) 15665..17890 - 2226 WP_028985935.1 P-loop NTPase fold protein -
JI426_RS27910 (17892) 17892..18980 - 1089 WP_028985936.1 transcriptional repressor PifC -
JI426_RS27915 (19560) 19560..19778 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JI426_RS27920 (19780) 19780..20085 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
JI426_RS27925 (20086) 20086..20892 + 807 Protein_16 site-specific integrase -
JI426_RS27930 (21666) 21666..21977 + 312 Protein_17 RepB family plasmid replication initiator protein -
JI426_RS27940 (23236) 23236..23682 + 447 Protein_19 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..118340 118340


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T186358 WP_001159868.1 NZ_CP067431:19780-20085 [Escherichia coli O22:H8]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T186358 NZ_CP089385:2018067-2018169 [Klebsiella variicola subsp. variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT186358 WP_000813634.1 NZ_CP067431:19560-19778 [Escherichia coli O22:H8]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT186358 NZ_CP089385:c2018175-2018031 [Klebsiella variicola subsp. variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References