Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 12997..13598 | Replicon | plasmid pO157 |
| Accession | NZ_CP067430 | ||
| Organism | Escherichia coli O22:H8 strain 154 | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | I2USK8 |
| Locus tag | JI426_RS27430 | Protein ID | WP_001216042.1 |
| Coordinates | 13218..13598 (+) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | I2USI4 |
| Locus tag | JI426_RS27425 | Protein ID | WP_001190710.1 |
| Coordinates | 12997..13218 (+) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JI426_RS27370 (JI426_005395) | 8686..9165 | + | 480 | WP_044869650.1 | hypothetical protein | - |
| JI426_RS27375 (JI426_005396) | 9338..9526 | + | 189 | WP_024174960.1 | hypothetical protein | - |
| JI426_RS27380 (JI426_005397) | 9528..9737 | + | 210 | WP_000951710.1 | hypothetical protein | - |
| JI426_RS27385 | 10127..10363 | + | 237 | Protein_18 | DUF551 domain-containing protein | - |
| JI426_RS27390 (JI426_005399) | 10360..10620 | + | 261 | WP_000969524.1 | hypothetical protein | - |
| JI426_RS27395 (JI426_005400) | 10620..10919 | + | 300 | WP_044869649.1 | ASCH domain-containing protein | - |
| JI426_RS27400 (JI426_005401) | 10906..11199 | + | 294 | WP_000267999.1 | hypothetical protein | - |
| JI426_RS27405 | 11224..11433 | + | 210 | WP_000190275.1 | hypothetical protein | - |
| JI426_RS27410 (JI426_005402) | 11430..12179 | + | 750 | WP_000196958.1 | hypothetical protein | - |
| JI426_RS27415 (JI426_005403) | 12163..12447 | + | 285 | WP_001142392.1 | hypothetical protein | - |
| JI426_RS27420 (JI426_005404) | 12535..12924 | + | 390 | WP_000506730.1 | S24 family peptidase | - |
| JI426_RS27425 (JI426_005405) | 12997..13218 | + | 222 | WP_001190710.1 | type II toxin-antitoxin system prevent-host-death family antitoxin | Antitoxin |
| JI426_RS27430 (JI426_005406) | 13218..13598 | + | 381 | WP_001216042.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| JI426_RS27435 (JI426_005407) | 13634..14728 | - | 1095 | WP_097506739.1 | hypothetical protein | - |
| JI426_RS27440 (JI426_005408) | 14718..15485 | - | 768 | WP_000446290.1 | hypothetical protein | - |
| JI426_RS27445 (JI426_005409) | 15492..16142 | - | 651 | WP_001108662.1 | DUF2829 domain-containing protein | - |
| JI426_RS27450 (JI426_005410) | 16157..16651 | - | 495 | WP_000640905.1 | dUTP diphosphatase | - |
| JI426_RS27455 (JI426_005411) | 16648..17124 | - | 477 | WP_000861171.1 | hypothetical protein | - |
| JI426_RS27460 (JI426_005412) | 17121..17465 | - | 345 | WP_164717617.1 | hypothetical protein | - |
| JI426_RS27465 (JI426_005413) | 17539..18567 | - | 1029 | WP_054192291.1 | tyrosine-type recombinase/integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..92972 | 92972 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13651.52 Da Isoelectric Point: 5.6406
>T186356 WP_001216042.1 NZ_CP067430:13218-13598 [Escherichia coli O22:H8]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI
Download Length: 381 bp
>T186356 NZ_CP089385:866076-866486 [Klebsiella variicola subsp. variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8147.13 Da Isoelectric Point: 4.7708
>AT186356 WP_001190710.1 NZ_CP067430:12997-13218 [Escherichia coli O22:H8]
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSELLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT186356 NZ_CP089385:865829-866095 [Klebsiella variicola subsp. variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | I2USK8 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A348ERB2 |