Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4061181..4062018 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag JI426_RS20250 Protein ID WP_000227784.1
Coordinates 4061476..4062018 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag JI426_RS20245 Protein ID WP_001297137.1
Coordinates 4061181..4061492 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS20220 (4056201) 4056201..4057148 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
JI426_RS20225 (4057170) 4057170..4059161 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
JI426_RS20230 (4059151) 4059151..4059765 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
JI426_RS20235 (4059765) 4059765..4060094 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
JI426_RS20240 (4060106) 4060106..4060996 + 891 WP_000971336.1 heme o synthase -
JI426_RS20245 (4061181) 4061181..4061492 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
JI426_RS20250 (4061476) 4061476..4062018 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
JI426_RS20255 (4062074) 4062074..4062337 - 264 Protein_3980 hypothetical protein -
JI426_RS20265 (4063596) 4063596..4064090 - 495 Protein_3982 tetratricopeptide repeat protein -
JI426_RS20270 (4064498) 4064498..4065862 + 1365 WP_249868948.1 MFS transporter -
JI426_RS20275 (4065990) 4065990..4066481 - 492 WP_001138904.1 nucleotide binding protein YajQ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T186350 WP_000227784.1 NZ_CP067426:4061476-4062018 [Escherichia coli O22:H8]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T186350 NZ_CP089383:c4606151-4605867 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATGGAGTCGGCCAGGTTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT186350 WP_001297137.1 NZ_CP067426:4061181-4061492 [Escherichia coli O22:H8]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT186350 NZ_CP089383:c4606383-4606141 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References