Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4027104..4027722 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JI426_RS20080 Protein ID WP_001291435.1
Coordinates 4027504..4027722 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JI426_RS20075 Protein ID WP_000344800.1
Coordinates 4027104..4027478 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS20065 (4022193) 4022193..4023386 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JI426_RS20070 (4023409) 4023409..4026558 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JI426_RS20075 (4027104) 4027104..4027478 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JI426_RS20080 (4027504) 4027504..4027722 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JI426_RS20085 (4027894) 4027894..4028445 + 552 WP_000102564.1 maltose O-acetyltransferase -
JI426_RS20090 (4028561) 4028561..4029031 + 471 WP_000136192.1 YlaC family protein -
JI426_RS20095 (4029195) 4029195..4030745 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JI426_RS20100 (4030787) 4030787..4031140 - 354 WP_000878141.1 DUF1428 family protein -
JI426_RS20110 (4031519) 4031519..4031830 + 312 WP_000409911.1 MGMT family protein -
JI426_RS20115 (4031861) 4031861..4032433 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186349 WP_001291435.1 NZ_CP067426:4027504-4027722 [Escherichia coli O22:H8]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186349 NZ_CP089383:c4525034-4524714 [Klebsiella pneumoniae]
ATGCACATTTCAACTGTACCGGCCAAGGTGCCGGTTTCGTCACGTCTGTCGCCCGTGCAGGTCTGGCAGCAACTTTTAAC
GTATCTGCTGGGTCACCATTACGGCCTGACGCTTAACGACACACCATTCCATGACGACGCGGCCATCGAAGAGCATATCG
ATGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGGTTT
AGCTGGCAGGAACAGACTCCGTTCCTGACCGCGACTGATATTCTCCGAGCCAGGCGAGCTACCGGATTAATAAATACTTA
A

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186349 WP_000344800.1 NZ_CP067426:4027104-4027478 [Escherichia coli O22:H8]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186349 NZ_CP089383:c4525394-4525071 [Klebsiella pneumoniae]
ATGTCAATCACACCACCTCATGAATGGGGTCTACAAAGCGATATCACCCCTCGATTTGGCGCTAGATTAGTTCAGGAAGG
CAACCGGCTGTATTACCTGGCTGACAGGGCTGGATTGACGGGAAGTTTTAGCCCAATGCACCTGCAGAAAATAGATCTGG
CTTTCCCGCTGTTTGTCGAACAACTGGAAGATATGCTGCATGCTGGCGAACTAAATCCACATCAGCAATGTCAGGTGAAA
ATTCAACTTGCGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGTGGTTACGTTTACATCTCTATTTATCCCAAACC
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References