Detailed information of TA system TA186337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1655606..1656231
• Replicon: chromosome
• Accession: NZ_CP067426
• Organism: Escherichia coli O22:H8 strain 154

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JI426_RS08225
• Protein ID: WP_000911330.1
• Coordinates: 1655833..1656231 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JI426_RS08220
• Protein ID: WP_000450524.1
• Coordinates: 1655606..1655833 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JI426_RS08195 (1651409)	1651409..1651879	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
JI426_RS08200 (1651879)	1651879..1652451	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JI426_RS08205 (1652597)	1652597..1653475	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JI426_RS08210 (1653492)	1653492..1654526	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
JI426_RS08215 (1654739)	1654739..1655452	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JI426_RS08220 (1655606)	1655606..1655833	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JI426_RS08225 (1655833)	1655833..1656231	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
JI426_RS08230 (1656378)	1656378..1657241	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
JI426_RS08235 (1657256)	1657256..1659271	+	2016	WP_032197281.1	tRNA cytosine(34) acetyltransferase TmcA	-
JI426_RS08240 (1659345)	1659345..1660043	+	699	WP_000679823.1	esterase	-
JI426_RS08245 (1660153)	1660153..1660353	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T186337 WP_000911330.1 NZ_CP067426:1655833-1656231 [Escherichia coli O22:H8]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T186337 NZ_CP089327:106521-107006 [Klebsiella pneumoniae]
GTGGGATATGTAACATCACCAGAACCGTTGTCTTCTTTTCATCAGGTGGCGGAGTTTATCAGCGGTGAAGCCGTGCTTGA
TGACTGGTTAAAGCAGAGGAGCCTCAAAAACCAGGCGACTGGAGCGGCCCGAACGTTTGTGGTGTGTAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAATCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATTCCTGTCATTATACTTGCCAGGCTTGCCGTCGATGTATCGTTCCGTGGAAAAGGGCTTGGTGCTGATTT
GCTTCATGACGCAGTGCTTCGTTGCTTTCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCACGCGCTTACGG
AAGAAGCCAAAAACTTCTATATTCATCATGGTTTCAAACCATCACAAAGTCAGCCACGGACATTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT186337 WP_000450524.1 NZ_CP067426:1655606-1655833 [Escherichia coli O22:H8]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT186337 NZ_CP089327:106267-106533 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTTAACCTCAGAGCCAAGGAATCCCAGCGAGCACTCATTGATGCTGCTGCTGAAATCCTTCA
CAAATCACGAACAGATTTCATTCTGGAAATGGCCTGTAAGGCTGCCGAGGATGTCATCCTTGACCGCCGCGTCTTTAATT
TTAACGATGAGCAATACGCCGAGTTCATCGATATGATTGATGCGCCGGTTGCAGATGATCCTGCTATCGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGATATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33