Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1229252..1229906 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag JI426_RS05935 Protein ID WP_000244772.1
Coordinates 1229252..1229659 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JI426_RS05940 Protein ID WP_000354046.1
Coordinates 1229640..1229906 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS05915 (1225209) 1225209..1226942 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
JI426_RS05920 (1226948) 1226948..1227658 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JI426_RS05925 (1227683) 1227683..1228579 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JI426_RS05930 (1228691) 1228691..1229212 + 522 WP_001055874.1 flavodoxin FldB -
JI426_RS05935 (1229252) 1229252..1229659 - 408 WP_000244772.1 protein YgfX Toxin
JI426_RS05940 (1229640) 1229640..1229906 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JI426_RS05945 (1230149) 1230149..1231129 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JI426_RS05950 (1231206) 1231206..1231865 - 660 WP_000250274.1 hemolysin III family protein -
JI426_RS05955 (1232029) 1232029..1232340 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JI426_RS05960 (1232385) 1232385..1233818 + 1434 WP_050864001.1 6-phospho-beta-glucosidase BglA -
JI426_RS05965 (1233875) 1233875..1234618 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T186336 WP_000244772.1 NZ_CP067426:c1229659-1229252 [Escherichia coli O22:H8]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T186336 NZ_CP089327:63211-63516 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT186336 WP_000354046.1 NZ_CP067426:c1229906-1229640 [Escherichia coli O22:H8]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT186336 NZ_CP089327:62991-63209 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References