Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 961398..962230 Replicon chromosome
Accession NZ_CP067426
Organism Escherichia coli O22:H8 strain 154

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2A2C6E0
Locus tag JI426_RS04650 Protein ID WP_000854716.1
Coordinates 961856..962230 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3U2KGT7
Locus tag JI426_RS04645 Protein ID WP_001285595.1
Coordinates 961398..961766 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JI426_RS04625 (959151) 959151..959969 + 819 WP_033801547.1 DUF932 domain-containing protein -
JI426_RS04630 (960061) 960061..960546 + 486 WP_000213718.1 antirestriction protein -
JI426_RS04635 (960562) 960562..961038 + 477 WP_001379208.1 RadC family protein -
JI426_RS04640 (961101) 961101..961340 + 240 WP_000692304.1 DUF987 domain-containing protein -
JI426_RS04645 (961398) 961398..961766 + 369 WP_001285595.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
JI426_RS04650 (961856) 961856..962230 + 375 WP_000854716.1 TA system toxin CbtA family protein Toxin
JI426_RS04655 (962227) 962227..962718 + 492 WP_000976843.1 DUF5983 family protein -
JI426_RS04660 (962730) 962730..962927 + 198 WP_000839249.1 DUF957 domain-containing protein -
JI426_RS04665 (963012) 963012..963857 + 846 WP_001280505.1 DUF4942 domain-containing protein -
JI426_RS04675 (964590) 964590..966843 + 2254 Protein_919 alpha-amylase family glycosyl hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13986.02 Da        Isoelectric Point: 7.2426

>T186334 WP_000854716.1 NZ_CP067426:961856-962230 [Escherichia coli O22:H8]
MKTLPDTHVREASRCPSPVIIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYLEAK

Download         Length: 375 bp

>T186334 NZ_CP089326:c22419-22003 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGCGCC
GCGGTGGATGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCGGCCGGCGCAGTCCTGGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13494.33 Da        Isoelectric Point: 6.3132

>AT186334 WP_001285595.1 NZ_CP067426:961398-961766 [Escherichia coli O22:H8]
VSDTLPGTTLPDDNHDRPWWGLPCTVTSCFGARLVQVGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT186334 NZ_CP089326:c22646-22416 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A2C6E0

Antitoxin

Source ID Structure
AlphaFold DB A0A3U2KGT7

References