Detailed information of TA system TA186330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 409569..410590
• Replicon: plasmid pTT6-2
• Accession: NZ_CP067422
• Organism: Skermanella sp. TT6

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: IGS68_RS33330
• Protein ID: WP_201083564.1
• Coordinates: 410018..410590 (+)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: IGS68_RS33325
• Protein ID: WP_201083118.1
• Coordinates: 409569..410018 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGS68_RS33290	405061..406449	-	1389	WP_201083105.1	DEAD/DEAH box helicase	-
IGS68_RS33295	406422..407306	-	885	WP_201083107.1	hypothetical protein	-
IGS68_RS33300	407716..407817	+	102	WP_201083109.1	hypothetical protein	-
IGS68_RS33305	407804..408172	+	369	WP_201083111.1	helix-turn-helix domain-containing protein	-
IGS68_RS33310	408372..408614	+	243	WP_201083113.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
IGS68_RS33315	408662..409120	+	459	WP_201083115.1	type II toxin-antitoxin system VapC family toxin	-
IGS68_RS33320	409159..409461	+	303	WP_201083116.1	helix-turn-helix domain-containing protein	-
IGS68_RS33325	409569..410018	+	450	WP_201083118.1	helix-turn-helix domain-containing protein	Antitoxin
IGS68_RS33330	410018..410590	+	573	WP_201083564.1	PIN domain-containing protein	Toxin
IGS68_RS33335	410849..412111	+	1263	WP_201070323.1	IS110 family transposase	-
IGS68_RS33340	412333..413181	-	849	WP_201083119.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..475996	475996
	-	flank	IS/Tn	-	-	403683..405041	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• SinI-like, DNA-binding domain (79-126)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21391.54 Da
• Isoelectric Point: 6.1447

>T186330 WP_201083564.1 NZ_CP067422:410018-410590 [Skermanella sp. TT6]
ILAGFTVVYDACVLYPAPLRDLLIELAAANLFRAKWTDAIHDEWIRNLLSNRPDLGIERLRRTRELMNTAVLDCLVKGYE
HLIPAVSLPDEDDRHVVAAAIHSRADAIVTFNLKDFPASELSRHDFEAVHPDEFIQSLFELDGAAVVIAAQRYHRKLKNP
PKSAGEYLDTPLRQSLPKTVASLRPFSEVI

Nucleotide

• Length: 573 bp

>T186330 NZ_CP089325:c4723758-4723474 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16722.12 Da
• Isoelectric Point: 5.6560

>AT186330 WP_201083118.1 NZ_CP067422:409569-410018 [Skermanella sp. TT6]
MNALIRRPESVVPTDEDARLAAELSRVLAASRENAALRVQLDDGRALTLPMAATRLLEQVLTEMAHGNAVTLIPVHAELT
TQEAADYLNVSRPYLVRLLEENFIPFHKVGTHRRVRFQDLKTYKEQADAKREMAMGDLVAQAQELDLGY

Nucleotide

• Length: 450 bp

>AT186330 NZ_CP089325:c4723990-4723748 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA