Detailed information of TA system TA186328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 473305..473969
• Replicon: plasmid pTT6-1
• Accession: NZ_CP067421
• Organism: Skermanella sp. TT6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IGS68_RS30000
• Protein ID: WP_201081818.1
• Coordinates: 473305..473712 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IGS68_RS30005
• Protein ID: WP_201081819.1
• Coordinates: 473709..473969 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IGS68_RS29970	468737..469663	-	927	WP_201081809.1	HlyD family efflux transporter periplasmic adaptor subunit	-
IGS68_RS29975	469660..470412	-	753	WP_201081811.1	ABC transporter ATP-binding protein	-
IGS68_RS29980	470409..471560	-	1152	WP_201081813.1	ABC transporter permease	-
IGS68_RS29985	471557..472165	-	609	WP_201081815.1	TetR/AcrR family transcriptional regulator	-
IGS68_RS29990	472296..472712	-	417	Protein_443	Tn3 family transposase	-
IGS68_RS29995	472768..472896	+	129	Protein_444	IS3 family transposase	-
IGS68_RS30000	473305..473712	-	408	WP_201081818.1	type II toxin-antitoxin system VapC family toxin	Toxin
IGS68_RS30005	473709..473969	-	261	WP_201081819.1	antitoxin	Antitoxin
IGS68_RS30010	474154..474554	-	401	Protein_447	integrase core domain-containing protein	-
IGS68_RS30015	474532..474762	-	231	WP_201082890.1	hypothetical protein	-
IGS68_RS30020	475303..476586	+	1284	WP_201081821.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiC1/vipB	1..821558	821558
	-	flank	IS/Tn	-	-	474532..474816	284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-45)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14865.04 Da
• Isoelectric Point: 5.2258

>T186328 WP_201081818.1 NZ_CP067421:c473712-473305 [Skermanella sp. TT6]
VIGWLLDTNAVIALTTRRSETLLRRVEATEPGALAVSAIVAHELYFGAYRSQKVDFNLETLRLLFTDIAILDLDREDARA
AGEIRADMKRQGTPIGPYDVLIAGQALARGLPLVTNNTAEFGRIAGLRLEDWTKT

Nucleotide

• Length: 408 bp

>T186328 NZ_CP089325:c4598441-4597908 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9823.88 Da
• Isoelectric Point: 5.1342

>AT186328 WP_201081819.1 NZ_CP067421:c473969-473709 [Skermanella sp. TT6]
MPHIAKVFQSGNSQAVRLPKDFRFAVDEVEVSREGDAVILRPRADSSRRWASLHAAIDRSLSDDFMAEGREQPDQQERPD
LDRAFQ

Nucleotide

• Length: 261 bp

>AT186328 NZ_CP089325:c4598718-4598452 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA