Detailed information of TA system TA186321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 230..798
• Replicon: plasmid pE31B
• Accession: NZ_CP067419
• Organism: Aeromonas media strain E31

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: JJL53_RS24665
• Protein ID: WP_101617266.1
• Coordinates: 230..523 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JJL53_RS24670
• Protein ID: WP_101617265.1
• Coordinates: 511..798 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL53_RS24665	230..523	-	294	WP_101617266.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJL53_RS24670	511..798	-	288	WP_101617265.1	CopG family ribbon-helix-helix protein	Antitoxin
JJL53_RS24675	1003..1632	+	630	WP_042044880.1	AAA family ATPase	-
JJL53_RS24680	1653..1850	+	198	WP_161508007.1	chromosome partitioning protein ParB	-
JJL53_RS24685	2688..2930	-	243	WP_103859311.1	plasmid replication protein RepB	-
JJL53_RS24690	3001..3351	-	351	WP_201035623.1	hypothetical protein	-
JJL53_RS24695	3577..3828	+	252	WP_041206019.1	DUF4160 domain-containing protein	-
JJL53_RS24700	3812..4234	+	423	WP_041206020.1	DUF2442 domain-containing protein	-
JJL53_RS24705	4394..5359	+	966	WP_201035624.1	replication initiation protein	-
JJL53_RS24710	5536..5778	-	243	WP_134997961.1	plasmid replication protein RepB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..15349	15349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-94)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10980.79 Da
• Isoelectric Point: 10.6871

>T186321 WP_101617266.1 NZ_CP067419:c523-230 [Aeromonas media]
MPPVIFAPAALRDLQRLREFLRPKSPQVAQRAATTIQLSLRQLAAQPSMGRPIEGLPEAFREWVIPFGDSGYLARYRIEP
DVIVVLAVRHQREVGYS

Nucleotide

• Length: 294 bp

>T186321 NZ_CP089325:343382-343750 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTATTAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11047.15 Da
• Isoelectric Point: 4.7091

>AT186321 WP_101617265.1 NZ_CP067419:c798-511 [Aeromonas media]
MPTQSKPVSIKLDADTQERIKRLATSRERSAHWLMREAISQYVEREELRETFHRDTLEAWQEFQETGQYATAQDVDQWLA
SWGTDAEGAPPACHQ

Nucleotide

• Length: 288 bp

>AT186321 NZ_CP089325:343164-343385 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA