186320

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2838661..2840375 Replicon chromosome
Accession NZ_CP067417
Organism Aeromonas media strain E31

Toxin (Protein)


Gene name pmenT Uniprot ID A0A6I4WWK8
Locus tag JJL53_RS13110 Protein ID WP_005331300.1
Coordinates 2838661..2839314 (+) Length 218 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID A0A6I4WU85
Locus tag JJL53_RS13115 Protein ID WP_041206015.1
Coordinates 2839311..2840375 (+) Length 355 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJL53_RS13085 2834477..2835424 - 948 WP_019706001.1 IS30-like element ISAs2 family transposase -
JJL53_RS13090 2835531..2835734 + 204 Protein_2526 tetracycline resistance MFS efflux pump -
JJL53_RS13095 2835993..2837261 - 1269 WP_041206016.1 IS4-like element ISAeme4 family transposase -
JJL53_RS13100 2837362..2838246 + 885 Protein_2528 IS30 family transposase -
JJL53_RS13105 2838251..2838394 + 144 WP_005331298.1 hypothetical protein -
JJL53_RS13110 2838661..2839314 + 654 WP_005331300.1 DUF4433 domain-containing protein Toxin
JJL53_RS13115 2839311..2840375 + 1065 WP_041206015.1 macro domain-containing protein Antitoxin
JJL53_RS13120 2840450..2843581 + 3132 WP_041206014.1 HsdR family type I site-specific deoxyribonuclease -
JJL53_RS13125 2843581..2844144 + 564 Protein_2533 M48 family metallopeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2844151..2845488 1337


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-216)

Antitoxin

Macro domain

(18-131)


Sequences

Toxin        


Download         Length: 218 a.a.        Molecular weight: 25275.87 Da        Isoelectric Point: 7.0173

>T186320 WP_005331300.1 NZ_CP067417:2838661-2839314 [Aeromonas media]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQSPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPRRENDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMNRVGVARPVYVRDGWYFR

Download         Length: 654 bp

>T186320 NZ_CP089321:46913-47263 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 355 a.a.        Molecular weight: 39588.70 Da        Isoelectric Point: 6.5002

>AT186320 WP_041206015.1 NZ_CP067417:2839311-2840375 [Aeromonas media]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Download         Length: 1065 bp

>AT186320 NZ_CP089321:46656-46919 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I4WWK8

Antitoxin

Source ID Structure
AlphaFold DB A0A6I4WU85

References