Detailed information of TA system TA186319

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2830124..2830643
• Replicon: chromosome
• Accession: NZ_CP067417
• Organism: Aeromonas media strain E31

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JJL53_RS13055
• Protein ID: WP_113722067.1
• Coordinates: 2830124..2830411 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S1GNF0
• Locus tag: JJL53_RS13060
• Protein ID: WP_026080514.1
• Coordinates: 2830401..2830643 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL53_RS13025	2825447..2825596	-	150	WP_158319061.1	hypothetical protein	-
JJL53_RS13030	2825635..2826009	-	375	WP_049832182.1	hypothetical protein	-
JJL53_RS13035	2826064..2827197	+	1134	WP_158319062.1	ATPase	-
JJL53_RS13040	2827520..2827891	+	372	Protein_2516	IS4 family transposase	-
JJL53_RS13045	2827920..2829017	-	1098	WP_123172271.1	ISAs1-like element ISAeme2 family transposase	-
JJL53_RS13050	2829733..2830074	-	342	WP_161508008.1	type II toxin-antitoxin system CcdA family antitoxin	-
JJL53_RS13055	2830124..2830411	-	288	WP_113722067.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJL53_RS13060	2830401..2830643	-	243	WP_026080514.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
JJL53_RS13065	2830901..2831170	-	270	WP_039062313.1	hypothetical protein	-
JJL53_RS13070	2831335..2832330	+	996	WP_201035141.1	hypothetical protein	-
JJL53_RS13075	2832717..2833349	-	633	WP_017411289.1	tetracycline resistance transcriptional repressor TetR(E)	-
JJL53_RS13080	2833430..2834446	+	1017	Protein_2524	Tet(A)/Tet(B)/Tet(C) family tetracycline efflux MFS transporter	-
JJL53_RS13085	2834477..2835424	-	948	WP_019706001.1	IS30-like element ISAs2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	tet(E)	-	2827520..2838246	10726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-62)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 9.9994

>T186319 WP_113722067.1 NZ_CP067417:c2830411-2830124 [Aeromonas media]
MTFELEFDPRAWKEWKKLGDTVRQQFKKKLAEVLERPRVEANRLHGLPDCYKIKLRGAGYRLVYQVQDDRVLVFVVAVGK
REREQVYLDAGSRLE

Nucleotide

• Length: 288 bp

>T186319 NZ_CP089319:498132-498473 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9057.37 Da
• Isoelectric Point: 4.6017

>AT186319 WP_026080514.1 NZ_CP067417:c2830643-2830401 [Aeromonas media]
MGTINLRIDDDLKTRSYAVLEKLGVTPSELLRQTLEYVASQERLPFRTTVVTEDEEALLALVRERLASPQKGIRVSLDDL

Nucleotide

• Length: 243 bp

>AT186319 NZ_CP089319:497902-498132 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S1GNF0