Detailed information of TA system TA186318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2116053..2117617
• Replicon: chromosome
• Accession: NZ_CP067417
• Organism: Aeromonas media strain E31

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A2S4TP05
• Locus tag: JJL53_RS09970
• Protein ID: WP_005329232.1
• Coordinates: 2116053..2117363 (-)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JJL53_RS09975
• Protein ID: WP_005329233.1
• Coordinates: 2117363..2117617 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL53_RS09955	2111479..2114049	+	2571	WP_041205615.1	DEAD/DEAH box helicase family protein	-
JJL53_RS09960	2114158..2115375	-	1218	WP_005330541.1	J domain-containing protein	-
JJL53_RS09970	2116053..2117363	-	1311	WP_005329232.1	type II toxin-antitoxin system HipA family toxin	Toxin
JJL53_RS09975	2117363..2117617	-	255	WP_005329233.1	helix-turn-helix domain-containing protein	Antitoxin
JJL53_RS09980	2118119..2118367	+	249	WP_010674978.1	hypothetical protein	-
JJL53_RS09985	2118788..2119039	-	252	WP_010674977.1	plasmid stabilization protein	-
JJL53_RS09990	2119118..2120500	-	1383	WP_201035452.1	Fic family protein	-
JJL53_RS09995	2120896..2122170	+	1275	WP_011191341.1	IS4-like element ISApu2 family transposase	-
JJL53_RS10000	2122181..2122531	+	351	Protein_1928	DUF2384 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2120896..2122170	1274

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 49096.31 Da
• Isoelectric Point: 7.1717

>T186318 WP_005329232.1 NZ_CP067417:c2117363-2116053 [Aeromonas media]
MASLTVYMNGYRVGTFTKATNGAHQFQYDESWVKLPGSRPISLSMPLRQQRYQGDEAYNFFDNLLPDNPEVRNRVVARYQ
ADSTQPFDLLSCIGQDSVGALQLVAQGRPVPDVKRIECKPLSDAELEQIMTSYQQGIPLGMVREEDDFRISIAGAQEKTA
LLYLDNRWCLPHAATPTTHIIKLPIGKIESHSYSIDLSQSVENEYLCTLIAKALGLPVPHCFIMQVGKVKALAVERFDRR
YATDRSWIMRLPQEDFCQVLNVPSARKYESHGGPGIADIMTTLLGSATPEQDRYLFMKSQVLFWLLAATDGHAKNFSVFI
EPEGRFRLTPFYDILSMYPAFGGKGLHPRDAKLAMGLTATKGKKYAIEQIFPRHFYQTAKAIGFEQVQMEKIISELTDNL
DEVISIVRQQLPDTFPTEIADSILGGLSSKAQRLKR

Nucleotide

• Length: 1311 bp

>T186318 NZ_CP089319:222066-222332 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9271.66 Da
• Isoelectric Point: 9.1571

>AT186318 WP_005329233.1 NZ_CP067417:c2117617-2117363 [Aeromonas media]
MKVTSAKQLSACLKDARLSQKLSQGKVAGKVGIRQDTVSSFELNPESTKLETLFKILAALELSLDIRPRTDPAENATPGW
KEEW

Nucleotide

• Length: 255 bp

>AT186318 NZ_CP089319:221803-222066 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A2S4TP05