Detailed information of TA system TA186313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2710262..2711865
• Replicon: chromosome
• Accession: NZ_CP067413
• Organism: Raoultella ornithinolytica strain TET32

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JJL43_RS12720
• Protein ID: WP_064367563.1
• Coordinates: 2710262..2711593 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: JJL43_RS12725
• Protein ID: WP_032717448.1
• Coordinates: 2711593..2711865 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL43_RS12705	2706136..2707050	-	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-
JJL43_RS12710	2707150..2708208	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
JJL43_RS12715	2708923..2710329	+	1407	WP_048023687.1	glycoside hydrolase family 10 protein	-
JJL43_RS12720	2710262..2711593	-	1332	WP_064367563.1	type II toxin-antitoxin system HipA family toxin	Toxin
JJL43_RS12725	2711593..2711865	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JJL43_RS12730	2712068..2712622	-	555	WP_044347941.1	RNA polymerase sigma factor	-
JJL43_RS12735	2712628..2713053	-	426	WP_032724724.1	hypothetical protein	-
JJL43_RS12740	2713186..2714139	-	954	WP_004861921.1	hypothetical protein	-
JJL43_RS12745	2714354..2714986	-	633	WP_064357056.1	class I SAM-dependent methyltransferase	-
JJL43_RS12750	2715006..2716247	-	1242	WP_004861912.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49313.63 Da
• Isoelectric Point: 6.7010

>T186313 WP_064367563.1 NZ_CP067413:c2711593-2710262 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGDQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T186313 NZ_CP089317:1803098-1803622 [Proteus mirabilis]
ATGACAATACCAAATTGGCACGAAGAAGCGATCAGTAAAAAACATAATAGAAATGGATTTAATTGTGGCGATGCAGTACT
AAACCAATTTCTGTATCGCCATGCAAGACAAAATCATGAGAATGGTAGTGCGAAAACATATTTAGCTATCAATAATAGTA
ATAATATTATATTAGGTTATTACAGCTTATGTCCTGCTTCTATTGAGTTCGAAAGAACGCCCGAATTGATCAAACATGGC
TTAGCGCGTCATGATATCCCTGTTTTTAAACTTGCACGACTAGCTACGGATCTATCCGTTCAAGGAAAAGGTTTGGGTGG
GCAATTATTGCTTGCAGCAGGAAGGCGTTGTCTTTCCGTTGCTAGTGAATTGGGAGGCGTTGCTTTACTTATAGATGCTA
AAAATGAACGAGTCGCTAATTGGTATATTAGCTACGGGGCAATACCATTATTAGATACCCCTCTTTCTTTATTAATTTCC
TTTAAAACCATTTATACTGCACTTTCAATGGCAAATAAAATATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT186313 WP_032717448.1 NZ_CP067413:c2711865-2711593 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT186313 NZ_CP089317:1802823-1803101 [Proteus mirabilis]
ATGGCTCAAGCACTAGTAGAAAACAGTGAACGAGTGTCAATTCGTATCACTACAGAAGATAAATCACTGCTGGTTCGTGC
TGCTAGTTTGGAAAATACAAACTTAACAGAGTTTGTACTTCGTAATATTGTACCTGTAGCTCGGCAAGTTATTGAAAATA
ATGAGCGTTTAAAATTATCAGAAAAAGACACATTACGTGTTATGGAACTTTTAGATAATCCACCAAAGCCTAATGCTAAA
CTACTTGCAGCGGCTTTTGCTCTACCGGACAATGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5