Detailed information of TA system TA186301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 67098..67645
• Replicon: chromosome
• Accession: NZ_CP067413
• Organism: Raoultella ornithinolytica strain TET32

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: JJL43_RS00315
• Protein ID: WP_088883551.1
• Coordinates: 67098..67406 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A808XYT1
• Locus tag: JJL43_RS00320
• Protein ID: WP_004869059.1
• Coordinates: 67409..67645 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJL43_RS00285	62277..62579	-	303	WP_015585486.1	PTS lactose/cellobiose transporter subunit IIA	-
JJL43_RS00290	62714..64039	-	1326	WP_004869064.1	PTS cellobiose transporter subunit IIC	-
JJL43_RS00295	64051..64365	-	315	WP_004869063.1	PTS sugar transporter subunit IIB	-
JJL43_RS00300	64660..65601	+	942	WP_015585487.1	LacI family transcriptional regulator	-
JJL43_RS00305	65696..65971	-	276	WP_160873538.1	hypothetical protein	-
JJL43_RS00310	66080..66982	-	903	WP_015585496.1	EamA family transporter	-
JJL43_RS00315	67098..67406	-	309	WP_088883551.1	CcdB family protein	Toxin
JJL43_RS00320	67409..67645	-	237	WP_004869059.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JJL43_RS00325	67751..69184	-	1434	WP_069476354.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
JJL43_RS00330	69209..70111	-	903	WP_201165357.1	N-acetylmuramic acid 6-phosphate etherase	-
JJL43_RS00335	70276..71439	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11794.61 Da
• Isoelectric Point: 6.7154

>T186301 WP_088883551.1 NZ_CP067413:c67406-67098 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLYDGI

Nucleotide

• Length: 309 bp

>T186301 NZ_CP089315:c5337432-5337145 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGTC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8898.08 Da
• Isoelectric Point: 5.6579

>AT186301 WP_004869059.1 NZ_CP067413:c67645-67409 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT186301 NZ_CP089315:c5337158-5336856 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A808XYT1