Detailed information of TA system TA186299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 5600863..5601502
• Replicon: chromosome
• Accession: NZ_CP067412
• Organism: Pseudomonas sp. HS6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T0K2L6
• Locus tag: JJN09_RS25445
• Protein ID: WP_064384144.1
• Coordinates: 5601320..5601502 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJN09_RS25440
• Protein ID: WP_249484265.1
• Coordinates: 5600863..5601288 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN09_RS25405 (JJN09_25375)	5596457..5597293	+	837	WP_249484262.1	FkbM family methyltransferase	-
JJN09_RS25415	5597843..5597986	-	144	WP_249490852.1	tyrosine-type recombinase/integrase	-
JJN09_RS25420 (JJN09_25390)	5598194..5599117	-	924	WP_249484263.1	hypothetical protein	-
JJN09_RS25425 (JJN09_25395)	5599448..5599891	-	444	WP_249484264.1	hypothetical protein	-
JJN09_RS25430	5600307..5600391	+	85	Protein_5032	transposase	-
JJN09_RS25435 (JJN09_25400)	5600530..5600814	-	285	WP_249490853.1	helix-turn-helix domain-containing protein	-
JJN09_RS25440 (JJN09_25405)	5600863..5601288	-	426	WP_249484265.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JJN09_RS25445 (JJN09_25410)	5601320..5601502	-	183	WP_064384144.1	type II toxin-antitoxin system HicA family toxin	Toxin
JJN09_RS25450 (JJN09_25415)	5601959..5602225	+	267	WP_249484266.1	DUF3077 domain-containing protein	-
JJN09_RS25455 (JJN09_25420)	5602284..5602994	-	711	WP_085647969.1	SDR family NAD(P)-dependent oxidoreductase	-
JJN09_RS25460 (JJN09_25425)	5603028..5603672	-	645	WP_249484267.1	DsbA family oxidoreductase	-
JJN09_RS25465 (JJN09_25430)	5603669..5603887	-	219	WP_007952610.1	4-oxalocrotonate tautomerase family protein	-
JJN09_RS25470 (JJN09_25435)	5604515..5605441	+	927	WP_085647967.1	LysR family transcriptional regulator	-
JJN09_RS25475 (JJN09_25440)	5605358..5605675	-	318	WP_007952614.1	AzlD domain-containing protein	-
JJN09_RS25480 (JJN09_25445)	5605654..5606370	-	717	WP_248744155.1	AzlC family ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6810.86 Da
• Isoelectric Point: 10.8173

>T186299 WP_064384144.1 NZ_CP067412:c5601502-5601320 [Pseudomonas sp. HS6]
MKYSEFRRWLKAQGVEFQTGKGSHFKVSLNGRSTVFPDHGAKEMGEGLRKSIIKQLGLKD

Nucleotide

• Length: 183 bp

>T186299 NZ_CP089315:4639649-4639867 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15516.58 Da
• Isoelectric Point: 4.3686

>AT186299 WP_249484265.1 NZ_CP067412:c5601288-5600863 [Pseudomonas sp. HS6]
MFEYALEVHEEPGSVWLSCAEIPEMHTAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPALTAAK
VGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Nucleotide

• Length: 426 bp

>AT186299 NZ_CP089315:4639248-4639622 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A3T0K2L6