Detailed information of TA system TA186297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5024473..5026199
• Replicon: chromosome
• Accession: NZ_CP067412
• Organism: Pseudomonas sp. HS6

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: JJN09_RS22775
• Protein ID: WP_249483888.1
• Coordinates: 5024473..5025117 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: JJN09_RS22780
• Protein ID: WP_249483889.1
• Coordinates: 5025114..5026199 (+)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN09_RS22750 (JJN09_22720)	5020275..5020802	+	528	WP_007990564.1	inorganic diphosphatase	-
JJN09_RS22755 (JJN09_22725)	5021024..5021761	+	738	WP_249483884.1	helix-turn-helix transcriptional regulator	-
JJN09_RS22760 (JJN09_22730)	5022228..5022659	+	432	WP_249483885.1	hypothetical protein	-
JJN09_RS22765 (JJN09_22735)	5022789..5023709	+	921	WP_249483886.1	restriction endonuclease	-
JJN09_RS22770 (JJN09_22740)	5023895..5024476	+	582	WP_249483887.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
JJN09_RS22775 (JJN09_22745)	5024473..5025117	+	645	WP_249483888.1	DUF4433 domain-containing protein	Toxin
JJN09_RS22780 (JJN09_22750)	5025114..5026199	+	1086	WP_249483889.1	macro domain-containing protein	Antitoxin
JJN09_RS22785 (JJN09_22755)	5026416..5027591	+	1176	WP_249483890.1	GGDEF domain-containing protein	-
JJN09_RS22790 (JJN09_22760)	5027578..5028390	-	813	WP_249483891.1	DUF2135 domain-containing protein	-
JJN09_RS22795 (JJN09_22765)	5028394..5030013	-	1620	WP_249483892.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 25026.51 Da
• Isoelectric Point: 6.2911

>T186297 WP_249483888.1 NZ_CP067412:5024473-5025117 [Pseudomonas sp. HS6]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCANSDVQAPQYVNIGNVDLIDKRRSRQVPIEPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRSNDEIVILVSSLYRVEELGLPFVFTNAHAYPSWTDYYNDLANLDQIDWSILQRRDFKRDPDDPRKM
ERYQAEALIYHHLPITGLLGIMCHTDAMKIRIEQDVAARGLTLPVHARPKWYFQ

Nucleotide

• Length: 645 bp

>T186297 NZ_CP089315:873666-874076 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTTGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGATATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGCCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 40733.70 Da
• Isoelectric Point: 5.5056

>AT186297 WP_249483889.1 NZ_CP067412:5025114-5026199 [Pseudomonas sp. HS6]
MIRFIQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRLYAAACKAKEVETGRMHVTATNELDGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLIENDVKSVAVPPLGAGNGGLKWPEVREQIVEVLGDLDADVLVFEPTNQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEKLPDTENPLNLQFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDAGISDVIWFDQERKSFLQTYLKTEAKEYAEALEQTAELIDGFESPFGMELLATVDWLLSQEGVSASVPAMREA
LKHWEGGAGAAVRKSNLFDDQSLDIALKRLTTSSFRPELLA

Nucleotide

• Length: 1086 bp

>AT186297 NZ_CP089315:873419-873685 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA