Detailed information of TA system TA186295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 941351..941870
• Replicon: chromosome
• Accession: NZ_CP067412
• Organism: Pseudomonas sp. HS6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JJN09_RS04325
• Protein ID: WP_249485898.1
• Coordinates: 941589..941870 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: J2NY45
• Locus tag: JJN09_RS04320
• Protein ID: WP_007960251.1
• Coordinates: 941351..941599 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN09_RS04305 (JJN09_04305)	937201..939375	-	2175	WP_249485895.1	cyclic di-3',5'-guanylate-activated glycosyltransferase NrfB	-
JJN09_RS04310 (JJN09_04310)	939372..940526	-	1155	WP_249485897.1	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)	-
JJN09_RS04315	940702..941036	-	335	Protein_854	thioredoxin	-
JJN09_RS04320 (JJN09_04315)	941351..941599	+	249	WP_007960251.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JJN09_RS04325 (JJN09_04320)	941589..941870	+	282	WP_249485898.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JJN09_RS04330 (JJN09_04325)	942171..943019	+	849	WP_064117896.1	transporter substrate-binding domain-containing protein	-
JJN09_RS04335 (JJN09_04330)	943071..943733	+	663	WP_249485899.1	amino acid ABC transporter permease	-
JJN09_RS04340 (JJN09_04335)	943743..944405	+	663	WP_011334481.1	amino acid ABC transporter permease	-
JJN09_RS04345 (JJN09_04340)	944402..945199	+	798	WP_249485901.1	amino acid ABC transporter ATP-binding protein	-
JJN09_RS04350 (JJN09_04345)	945231..945620	+	390	WP_007912330.1	RidA family protein	-
JJN09_RS04355 (JJN09_04350)	945947..946711	+	765	WP_249485902.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10948.79 Da
• Isoelectric Point: 10.4217

>T186295 WP_249485898.1 NZ_CP067412:941589-941870 [Pseudomonas sp. HS6]
MTYELEFSEKAWKEWKKLDANLKEQLKKKLQQRLENPHVPADRLRGLGSAYKIKLRNVGYRMVYRVKDEVLVVTVIAIGK
REGGDVYRVATER

Nucleotide

• Length: 282 bp

>T186295 NZ_CP089315:40255-40596 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8902.28 Da
• Isoelectric Point: 4.5183

>AT186295 WP_007960251.1 NZ_CP067412:941351-941599 [Pseudomonas sp. HS6]
MTHIVLSQVVASISELKKNPMGTVAAGGGDSVAILNRNEPAFYCVPAKAYEALMNRLEDLELIALCKERENDPTIKVSID
DL

Nucleotide

• Length: 249 bp

>AT186295 NZ_CP089315:40606-40905 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Structures

Antitoxin

• AlphaFold DB: J2NY45