Detailed information of TA system TA186294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 765947..766826
• Replicon: chromosome
• Accession: NZ_CP067412
• Organism: Pseudomonas sp. HS6

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JJN09_RS03660
• Protein ID: WP_249485736.1
• Coordinates: 766473..766826 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJN09_RS03655
• Protein ID: WP_249485734.1
• Coordinates: 765947..766420 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJN09_RS03640 (JJN09_03640)	762070..762891	-	822	WP_249485727.1	phosphatase PAP2 family protein	-
JJN09_RS03645 (JJN09_03645)	762888..763559	-	672	WP_249485729.1	histidine phosphatase family protein	-
JJN09_RS03650 (JJN09_03650)	763813..765732	+	1920	WP_249485732.1	methyl-accepting chemotaxis protein	-
JJN09_RS03655 (JJN09_03655)	765947..766420	-	474	WP_249485734.1	transcriptional regulator	Antitoxin
JJN09_RS03660 (JJN09_03660)	766473..766826	-	354	WP_249485736.1	type II toxin-antitoxin system HigB family toxin	Toxin
JJN09_RS03665 (JJN09_03665)	767006..768379	-	1374	WP_249485737.1	DUF1329 domain-containing protein	-
JJN09_RS03670 (JJN09_03670)	768543..770501	-	1959	WP_249485739.1	DUF1302 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13826.19 Da
• Isoelectric Point: 10.5519

>T186294 WP_249485736.1 NZ_CP067412:c766826-766473 [Pseudomonas sp. HS6]
MRVITKAAVARAIETHSQWRDPLTLWLTIFDRASLRFESFEKLRKDWAMASGWNVDRIPHSKLRITSKKGPLDIYVFDIK
KNECRVITWINPKIGTIYIKEILPHAGYDKWWKGDVK

Nucleotide

• Length: 354 bp

>T186294 NZ_CP089313:4549399-4549710 [Salmonella enterica subsp. enterica serovar Indiana]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17285.68 Da
• Isoelectric Point: 4.7075

>AT186294 WP_249485734.1 NZ_CP067412:c766420-765947 [Pseudomonas sp. HS6]
MKRNKVEASGLDSFIADISPMLDGLGDQIATLSKLLSACHDPIKSDADLQGRMALIDELYSHADSEDHAAARFAELVADR
VYEYETETVLIPYSTQSEALAFLISERGVKQKDLSGIATQSAVSEILNNKRKMTVAQIKGFAEFFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT186294 NZ_CP089313:4549707-4550153 [Salmonella enterica subsp. enterica serovar Indiana]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG