Detailed information of TA system TA186291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 91673..92316
• Replicon: plasmid pXJW9B263-1
• Accession: NZ_CP067400
• Organism: Escherichia coli strain XJW9B263

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D7Y5A6
• Locus tag: JJF91_RS23820
• Protein ID: WP_001044765.1
• Coordinates: 91673..92089 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: JJF91_RS23825
• Protein ID: WP_001261282.1
• Coordinates: 92086..92316 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJF91_RS23790 (JJF91_23675)	86932..87417	+	486	WP_000045622.1	conjugative transfer protein MobI(A/C)	-
JJF91_RS23795 (JJF91_23680)	87414..87779	+	366	WP_001515713.1	hypothetical protein	-
JJF91_RS23800 (JJF91_23685)	87880..88101	-	222	Protein_96	transposase	-
JJF91_RS23805 (JJF91_23690)	88155..88849	+	695	WP_085949432.1	IS1-like element IS1N family transposase	-
JJF91_RS23810 (JJF91_23695)	89030..90052	-	1023	WP_000361404.1	helicase UvrD	-
JJF91_RS23815 (JJF91_23700)	90037..91599	-	1563	WP_001128475.1	AAA family ATPase	-
JJF91_RS23820 (JJF91_23705)	91673..92089	-	417	WP_001044765.1	type II toxin-antitoxin system VapC family toxin	Toxin
JJF91_RS23825 (JJF91_23710)	92086..92316	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JJF91_RS24885	92861..93151	+	291	WP_021570624.1	hypothetical protein	-
JJF91_RS23835 (JJF91_23720)	93309..93917	+	609	WP_001375052.1	hypothetical protein	-
JJF91_RS23840 (JJF91_23725)	93985..94767	+	783	WP_011251351.1	site-specific integrase	-
JJF91_RS23845 (JJF91_23730)	94905..95180	-	276	WP_000239529.1	hypothetical protein	-
JJF91_RS23850 (JJF91_23735)	95174..95818	-	645	WP_000633913.1	division plane positioning ATPase MipZ	-
JJF91_RS23855 (JJF91_23740)	96047..97018	+	972	WP_011251350.1	plasmid segregation protein ParM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14	-	1..138776	138776

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15148.60 Da
• Isoelectric Point: 7.1082

>T186291 WP_001044765.1 NZ_CP067400:c92089-91673 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Nucleotide

• Length: 417 bp

>T186291 NZ_CP089313:3629569-3629820 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCGGGATACTTACAAAACCCACATGACCACCTATTACTCGCGCATCCCCAGCCTGCACCTTAAGGGCGACTGGCTGGC
AGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGATCTCCGAAGGGTGTTTGACTCTGATTGTGGAGACAGACG
AAGTGAGGGATCTACGGAGGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCACATTAAGGCGGGGGTATATGATGTGGTG
AACGGGAATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT186291 WP_001261282.1 NZ_CP067400:c92316-92086 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT186291 NZ_CP089313:c3629810-3629585 [Salmonella enterica subsp. enterica serovar Indiana]
TTCACCACATCATATACCCCCGCCTTAATGTGCTTCATCGACTTTTTCACCTGATAAAGCTCCCTCCGTAGATCCCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCTTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCAGGCTGGGGATGCGCGAGTAATAGGTGGTCATGTGGGTTT

Structures

Toxin

• AlphaFold DB: A0A2H9AY90

Antitoxin

• AlphaFold DB: A0A0H3GZG3