Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 70753..71551 Replicon plasmid pXJW9B263-1
Accession NZ_CP067400
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name ataT Uniprot ID A0A2L1KTV6
Locus tag JJF91_RS23705 Protein ID WP_023135659.1
Coordinates 71030..71551 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag JJF91_RS23700 Protein ID WP_001711191.1
Coordinates 70753..71022 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS23675 (JJF91_23560) 66180..67520 - 1341 WP_023135655.1 DnaB-like helicase C-terminal domain-containing protein -
JJF91_RS23680 (JJF91_23565) 67581..68306 - 726 WP_023135656.1 hypothetical protein -
JJF91_RS23685 (JJF91_23570) 68589..69356 + 768 WP_001711193.1 hypothetical protein -
JJF91_RS23690 (JJF91_23575) 69409..69762 - 354 WP_161953766.1 hypothetical protein -
JJF91_RS23695 (JJF91_23580) 69768..70433 - 666 WP_023135658.1 AAA family ATPase -
JJF91_RS23700 (JJF91_23585) 70753..71022 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
JJF91_RS23705 (JJF91_23590) 71030..71551 + 522 WP_023135659.1 GNAT family N-acetyltransferase Toxin
JJF91_RS23710 (JJF91_23595) 71720..71971 - 252 WP_023135660.1 hypothetical protein -
JJF91_RS23715 (JJF91_23600) 71974..72741 - 768 WP_023135661.1 hypothetical protein -
JJF91_RS23720 (JJF91_23605) 72764..74338 - 1575 Protein_79 hypothetical protein -
JJF91_RS23725 (JJF91_23610) 74372..75628 - 1257 WP_023135663.1 phage terminase large subunit -
JJF91_RS23730 (JJF91_23615) 75631..76272 - 642 WP_023135664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..138776 138776


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19382.22 Da        Isoelectric Point: 8.2535

>T186290 WP_023135659.1 NZ_CP067400:71030-71551 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRHVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T186290 NZ_CP089313:3427737-3427955 [Salmonella enterica subsp. enterica serovar Indiana]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT186290 WP_001711191.1 NZ_CP067400:70753-71022 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp

>AT186290 NZ_CP089313:3427335-3427709 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1KTV6

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References