Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4345686..4346183 Replicon chromosome
Accession NZ_CP067399
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JJF91_RS21175 Protein ID WP_001173343.1
Coordinates 4345686..4345859 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JJF91_RS21180 Protein ID WP_000398619.1
Coordinates 4345887..4346183 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS21165 (4342356) 4342356..4343813 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
JJF91_RS21170 (4344050) 4344050..4345567 + 1518 WP_001295074.1 lysine--tRNA ligase -
JJF91_RS21175 (4345686) 4345686..4345859 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JJF91_RS21180 (4345887) 4345887..4346183 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JJF91_RS21185 (4346410) 4346410..4346682 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
JJF91_RS21190 (4346694) 4346694..4346924 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JJF91_RS21195 (4347105) 4347105..4348736 + 1632 WP_001216477.1 sensor histidine kinase -
JJF91_RS21200 (4348733) 4348733..4349452 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T186288 WP_001173343.1 NZ_CP067399:c4345859-4345686 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T186288 NZ_CP089313:2102703-2102806 [Salmonella enterica subsp. enterica serovar Indiana]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT186288 WP_000398619.1 NZ_CP067399:c4346183-4345887 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT186288 NZ_CP089313:c2102808-2102663 [Salmonella enterica subsp. enterica serovar Indiana]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References