Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2327899..2328425 Replicon chromosome
Accession NZ_CP067399
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JJF91_RS11475 Protein ID WP_000323025.1
Coordinates 2328138..2328425 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JJF91_RS11470 Protein ID WP_000534858.1
Coordinates 2327899..2328138 (+) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS11440 (2323258) 2323258..2323605 - 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
JJF91_RS11445 (2323602) 2323602..2324006 - 405 WP_000839179.1 transposase -
JJF91_RS11450 (2324228) 2324228..2324649 + 422 Protein_2249 hypothetical protein -
JJF91_RS11455 (2325305) 2325305..2326327 + 1023 Protein_2250 ISNCY family transposase -
JJF91_RS24725 (2326448) 2326448..2326597 + 150 WP_011443592.1 protein YdfW -
JJF91_RS11460 (2327034) 2327034..2327366 - 333 WP_001301033.1 FlxA-like family protein -
JJF91_RS11465 (2327569) 2327569..2327874 - 306 WP_001326990.1 protein YdfV -
JJF91_RS11470 (2327899) 2327899..2328138 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JJF91_RS11475 (2328138) 2328138..2328425 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JJF91_RS11480 (2328497) 2328497..2328652 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JJF91_RS11485 (2328869) 2328869..2329120 + 252 WP_000980994.1 protein Rem -
JJF91_RS11490 (2329187) 2329187..2329465 + 279 WP_012304870.1 hypothetical protein -
JJF91_RS11495 (2329467) 2329467..2330516 + 1050 WP_001265199.1 DUF968 domain-containing protein -
JJF91_RS11500 (2330530) 2330530..2331000 + 471 Protein_2260 antitermination protein -
JJF91_RS11505 (2331055) 2331055..2331752 + 698 WP_106423973.1 IS1 family transposase -
JJF91_RS11510 (2331766) 2331766..2331870 - 105 Protein_2262 putative zinc-binding protein YnfU -
JJF91_RS11515 (2332090) 2332090..2332305 + 216 WP_000839590.1 phage lysis protein EssD -
JJF91_RS11520 (2332310) 2332310..2332621 + 312 WP_000189916.1 YdfR family protein -
JJF91_RS11525 (2332618) 2332618..2333151 + 534 WP_001092971.1 lysozyme -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2305596..2380271 74675
- flank IS/Tn - - 2331459..2331752 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T186281 WP_000323025.1 NZ_CP067399:2328138-2328425 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T186281 NZ_CP089312:5425611-5425874 [Streptomyces sp. GQFP]
GTGAGTGAGTACCGCACTGTTTTCCGGCCCGAGGCCCAGGCCGAGCTCCGCAAGATCCCCCGTGACGCGGCGCTCCGCAT
CCTCGCCAAGCTGACGGAGCTGGAGACAGATCCGCTCGGCTTCAGCACCACGGCGCTCGTCTCCCAGCCGGAGAGACGCC
GACTGCGCGTCGGCGACTACCGCGTCATCTACACGATCGACAACGGGGAACTTGTTGTGTGGGTCGTTCACGTCGGGCAC
CGGTCCACGGTCTACGGCACCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT186281 WP_000534858.1 NZ_CP067399:2327899-2328138 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT186281 NZ_CP089312:5425313-5425579 [Streptomyces sp. GQFP]
ATGACGCAGATGCCCATAGAGTCCATCCGCGCTGTCCGTGCTCACCTGGCCGAGGTGGTCGAGCGGGCCGATCGGGACGA
CATGCCGACGGTCATCACCCGGCGGGGCAAGGAAGTGGCCGCCGTCGTGTCGATCGAGGTTCTGCGCAGGTACCAGGAGT
GGGAAGAGCGCGAGATCAACCGGATCATCGACGAGCGCATGGCGAATCGGGTCGCCGGTGTCCCGATCGAGGACGTCATG
CGGGAGACGCTGGCGCGCGGTGAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References