Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1289168..1289727 Replicon chromosome
Accession NZ_CP067399
Organism Escherichia coli strain XJW9B263

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag JJF91_RS06315 Protein ID WP_000421257.1
Coordinates 1289452..1289727 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag JJF91_RS06310 Protein ID WP_001178089.1
Coordinates 1289168..1289452 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJF91_RS06285 (1284843) 1284843..1285415 + 573 Protein_1235 IS1380-like element ISEcp1 family transposase -
JJF91_RS06290 (1285472) 1285472..1286440 - 969 WP_013362812.1 IS5-like element IS903B family transposase -
JJF91_RS06295 (1286475) 1286475..1287350 - 876 WP_001617865.1 extended-spectrum class A beta-lactamase CTX-M-14 -
JJF91_RS06300 (1287600) 1287600..1287887 - 288 Protein_1238 transposase -
JJF91_RS06305 (1287953) 1287953..1288657 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JJF91_RS06310 (1289168) 1289168..1289452 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
JJF91_RS06315 (1289452) 1289452..1289727 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JJF91_RS06325 (1290539) 1290539..1290907 + 369 Protein_1243 colicin-like pore-forming protein -
JJF91_RS06330 (1290929) 1290929..1291264 - 336 WP_001080730.1 colicin 1A immunity protein -
JJF91_RS06335 (1291393) 1291393..1291740 + 348 WP_000142451.1 DUF6404 family protein -
JJF91_RS06340 (1291827) 1291827..1292531 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
JJF91_RS06345 (1292556) 1292556..1293080 + 525 WP_050195267.1 recombinase family protein -
JJF91_RS06350 (1293157) 1293157..1294548 + 1392 WP_000085084.1 SIR2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element floR / tet(A) / aph(6)-Id / mph(A) / cmlA1 / qacE / blaCTX-M-14 / aac(3)-IId / fosA3 / sul2 - 1200628..1423795 223167
- inside IScluster/Tn floR / tet(A) / aph(6)-Id / mph(A) / cmlA1 / qacE / blaCTX-M-14 - 1268703..1299829 31126


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T186270 WP_000421257.1 NZ_CP067399:1289452-1289727 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T186270 NZ_CP089309:c4665738-4665268 [Thiocapsa bogorovii]
ATGCGGGCGCTCTTGGACGTCAATGTGCTCATCGCGTTGCTGGATGCGAGCCACATGCATCACGACACCGCAATGCGCTG
GTTGGAGCGTGAGCAGGCCCATGGTTGGGCATCCGGTCCGATCACGCAAAACGGCTGTATCCGGATCATGTCTCAGCCCG
CCTACCCGGCGCCGTTGCCCGCGGCCGCGGTGGCGGCGCGGTTGGCCGAGGCCGTGACAGGGCCGGATCATCAGTTCTGG
CCGGACGACCTGAACCCGGTTGTCGAAAAGACCCTGACCTGTGGACAAGTGCTGGGCCATCGCCAAGTCACCGACGCCTA
CCTGTTGGCGCTGGCCGTCAAGAACGGTGGGCGCTTCGTCACCTTACGATGCACGCGTGGCTTTGCAGACCGTCTTCGGC
GCCGGGAAGGAGCACCTCACCGTCATCGCGTGAAGAGGTCCTGGATGCGGGGCCGGATGAGCGCACGTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT186270 WP_001178089.1 NZ_CP067399:1289168-1289452 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT186270 NZ_CP089309:c4665980-4665738 [Thiocapsa bogorovii]
ATGATGCGAACGACCTTGGATATTGACGACGATGTGCTCGCCGCGGCGAAGGAGCGCGCTCGGTTGCGGAAGGTCTCCGC
CGGCAAGATGGTGTCCGATCTGCTGCGCCAAGCGCTTTCCGGCGGCACGGCACAGGACGAGACGAGGCAACGTGGGGTGG
CCGGATTTCGGCCCTTCGATGCTCGCGGCGTCATCGTCACGAACGACGACATCGATACCCTTAGAGACCGTGAAGGCGTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References