Detailed information of TA system TA186243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 528034..528654
• Replicon: chromosome
• Accession: NZ_CP067397
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain LB5000

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: JJB81_RS02395
• Protein ID: WP_001280991.1
• Coordinates: 528034..528252 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: JJB81_RS02400
• Protein ID: WP_000344807.1
• Coordinates: 528280..528654 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB81_RS02355 (523259)	523259..523828	+	570	WP_000779803.1	YbaY family lipoprotein	-
JJB81_RS02360 (523861)	523861..524250	-	390	WP_000961285.1	MGMT family protein	-
JJB81_RS02370 (524481)	524481..526031	-	1551	WP_210138930.1	EAL domain-containing protein	-
JJB81_RS02375 (526256)	526256..526516	+	261	WP_000801415.1	type B 50S ribosomal protein L31	-
JJB81_RS02380 (526522)	526522..526662	+	141	WP_001197749.1	type B 50S ribosomal protein L36	-
JJB81_RS02385 (526718)	526718..527188	-	471	WP_000136181.1	YlaC family protein	-
JJB81_RS02390 (527313)	527313..527855	-	543	Protein_468	maltose O-acetyltransferase	-
JJB81_RS02395 (528034)	528034..528252	-	219	WP_001280991.1	HHA domain-containing protein	Toxin
JJB81_RS02400 (528280)	528280..528654	-	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
JJB81_RS02405 (529150)	529150..532299	-	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
JJB81_RS02410 (532322)	532322..533515	-	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T186243 WP_001280991.1 NZ_CP067397:c528252-528034 [Salmonella enterica subsp. enterica serovar Typhimurium]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T186243 NZ_CP089301:c5478145-5477822 [Novosphingobium kaempferiae]
GTGAAACGCGGCGATCTCGTGACGATCGCGGCGCCAGGCGATTTCGGCAAGCCGCGCCCGGCCCTCGTCGTCCAGTCCGA
CCGCTTCGATGCGACCGGCACGCTGACGGTGCTGCTGCTCTCCGGCACGCCGGTGGACGCGCCGCTGATCCGCGTGCCCG
TGGCGCCGACACCCGATAACGGCCTGCGCAAGCCCTCGCAGGTCATGGTCGACAAGGCCATGTCGGTGAAACGCGAGCGG
ATCGGCGCAACCATCGGTCAACTGGACGCCGAAACGATGCTGTCGGTGACGCGTTCGCTAGCGGTCTTCCTCGACATTGC
GTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT186243 WP_000344807.1 NZ_CP067397:c528654-528280 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT186243 NZ_CP089301:c5478363-5478142 [Novosphingobium kaempferiae]
ATGCCCGCACCTGTCAGCGAACGCGTCCGGAAGCGCCGCGATGCGCTGCGTGCCGCGGGGCTGCGTCCGATCCAGATCTG
GGTGCCGGACACGCGCAGGCCCGGGTTTGCCGAAGAATGCCGCAGGCAGGCCCTCGCCGTCGCTGCTGCCGATACCACCG
ATGCCGACCTCGACGACTTCCTTGACGCCGCGCTCGTCGACCTGGACGCCGAAGACGAGTGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0