Detailed information of TA system TA186221

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 833527..834184
• Replicon: chromosome
• Accession: NZ_CP067391
• Organism: Klebsiella grimontii strain 5512.56

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A9E1DA00
• Locus tag: JJJ11_RS06385
• Protein ID: WP_024358614.1
• Coordinates: 833774..834184 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: H3N295
• Locus tag: JJJ11_RS06380
• Protein ID: WP_004124953.1
• Coordinates: 833527..833793 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJJ11_RS06355 (JJJ11_06355)	828970..829698	-	729	WP_024358621.1	MurR/RpiR family transcriptional regulator	-
JJJ11_RS06360 (JJJ11_06360)	829749..830060	+	312	WP_004133599.1	N(4)-acetylcytidine aminohydrolase	-
JJJ11_RS06365 (JJJ11_06365)	830223..830882	+	660	WP_004124978.1	hemolysin III family protein	-
JJJ11_RS06370 (JJJ11_06370)	831068..832048	-	981	WP_049130515.1	IS110 family transposase	-
JJJ11_RS06375 (JJJ11_06375)	832299..833282	-	984	WP_024358615.1	tRNA-modifying protein YgfZ	-
JJJ11_RS06380 (JJJ11_06380)	833527..833793	+	267	WP_004124953.1	FAD assembly factor SdhE	Antitoxin
JJJ11_RS06385 (JJJ11_06385)	833774..834184	+	411	WP_024358614.1	protein YgfX	Toxin
JJJ11_RS06390 (JJJ11_06390)	834193..834714	-	522	WP_024358613.1	flavodoxin FldB	-
JJJ11_RS06395 (JJJ11_06395)	834836..835732	+	897	WP_209573824.1	site-specific tyrosine recombinase XerD	-
JJJ11_RS06400 (JJJ11_06400)	835755..836468	+	714	WP_004134425.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
JJJ11_RS06405 (JJJ11_06405)	836474..838207	+	1734	WP_098066860.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	831068..832096	1028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16135.97 Da
• Isoelectric Point: 10.9455

>T186221 WP_024358614.1 NZ_CP067391:833774-834184 [Klebsiella grimontii]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWEIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Nucleotide

• Length: 411 bp

>T186221 NZ_CP089272:c4721803-4721489 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.88 Da
• Isoelectric Point: 5.1296

>AT186221 WP_004124953.1 NZ_CP067391:833527-833793 [Klebsiella grimontii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT186221 NZ_CP089272:c4722039-4721806 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H3L9