Detailed information of TA system TA186219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 25823..26559
• Replicon: plasmid p5512.56-1
• Accession: NZ_CP067390
• Organism: Klebsiella grimontii strain 5512.56

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: JJJ11_RS01295
• Protein ID: WP_003026803.1
• Coordinates: 26077..26559 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JJJ11_RS01290
• Protein ID: WP_003026799.1
• Coordinates: 25823..26089 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJJ11_RS01245 (JJJ11_01245)	21885..22247	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
JJJ11_RS01250 (JJJ11_01250)	22297..22647	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
JJJ11_RS01255 (JJJ11_01255)	23005..23274	+	270	WP_019706036.1	hypothetical protein	-
JJJ11_RS01260 (JJJ11_01260)	23262..23837	+	576	WP_004152103.1	hypothetical protein	-
JJJ11_RS01265 (JJJ11_01265)	23868..24362	+	495	WP_004152104.1	DNA-binding protein	-
JJJ11_RS01270 (JJJ11_01270)	24406..24774	+	369	WP_004152105.1	hypothetical protein	-
JJJ11_RS01275 (JJJ11_01275)	24808..25011	+	204	WP_004152106.1	HHA domain-containing protein	-
JJJ11_RS01280 (JJJ11_01280)	25060..25317	+	258	WP_004152107.1	hypothetical protein	-
JJJ11_RS01285 (JJJ11_01285)	25393..25647	+	255	WP_004152108.1	hypothetical protein	-
JJJ11_RS01290 (JJJ11_01290)	25823..26089	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JJJ11_RS01295 (JJJ11_01295)	26077..26559	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
JJJ11_RS01300 (JJJ11_01300)	26718..28250	-	1533	WP_009310015.1	IS3-like element ISKpn38 family transposase	-
JJJ11_RS01305 (JJJ11_01305)	28362..29765	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JJJ11_RS01310 (JJJ11_01310)	29794..30426	-	633	WP_001567369.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..232127	232127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T186219 WP_003026803.1 NZ_CP067390:26077-26559 [Klebsiella grimontii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T186219 NZ_CP089272:4181527-4181745 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT186219 WP_003026799.1 NZ_CP067390:25823-26089 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT186219 NZ_CP089272:4181127-4181501 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66